Identification and Characterization of the RNase lit Gene of the Tiger Frog Virus
Abstract: From the tiger frog virus(TFV)genome,an open reading frame(ORF),whose deduced amino acid sequence(aa)shows identity with RNase 111。was identified.The ORF has 1 113bp long and codes a putative protein of 371 aa with a predicted molecular mass of 40.47kD and a G+ C content of 56.63% . Stem loops and short dyad sequences appear in the downstream of the translation stop codon.Compared with the RNase 111 S found in TFV and other species.the higher homology among TFV and the tWO iridovirus is present,and TFV has lower identity with other species, especially yeast and rhabditida.