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Volume 18 Issue 1
February 2003
    Citation: ZHANG Ying, BAI Xue-fan“, HUANG Chang-xing, SUN Yong—tao, PAN Lei, LI Guang—yu, WANG Lin—XH. HIV-1 Coreceptor Chmokine Bicistronic expression vector Transfection .VIROLOGICA SINICA, 2003, 18(1) : 23-26.
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    HIV-1 Coreceptor Chmokine Bicistronic expression vector Transfection

    • 1.

      The Center of Diagnosis and Treotment for Infectious Diseases ofPLA，Tangdu Hospital，FMMU，Xi'an710038，China

    • Available online: 02 February 2003
    • In order to construct bicistronic expression vector of RANTES and SDF-l,the ligands of HIV-1 principal coreceptors,RANTES-KDEL were amplified from pCMV-R-K by PCR and ligased with eukaryotic expression vector pCMV-S/K. The construction of pCMV-R-K-S-K was confirmed by enzymatic digestion and sequencing. Then pCMV-R-K-S-K transfection into HeLa cells was carried out by lipofectin. RAN TES and SDF-1 were showed expressed in HeLa cells by indirect immumofluo-rescence. The results show that pCMV-R-K-S-K was constructed and expressed in cell line HeLa successfully, which will contribute to gene therapy of HIV-1.
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      HIV-1 Coreceptor Chmokine Bicistronic expression vector Transfection

      • 1. The Center of Diagnosis and Treotment for Infectious Diseases ofPLA，Tangdu Hospital，FMMU，Xi'an710038，China

      Abstract: In order to construct bicistronic expression vector of RANTES and SDF-l,the ligands of HIV-1 principal coreceptors,RANTES-KDEL were amplified from pCMV-R-K by PCR and ligased with eukaryotic expression vector pCMV-S/K. The construction of pCMV-R-K-S-K was confirmed by enzymatic digestion and sequencing. Then pCMV-R-K-S-K transfection into HeLa cells was carried out by lipofectin. RAN TES and SDF-1 were showed expressed in HeLa cells by indirect immumofluo-rescence. The results show that pCMV-R-K-S-K was constructed and expressed in cell line HeLa successfully, which will contribute to gene therapy of HIV-1.

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