Citation: LIU Guang-qing, LIU Zai-xin, ZHANG XiaIl-sheng, CHANG Hui-yun, GUO Hui-chen, LI Dong, U Xiang-tao, XIE Qing-ge. The Sequence Analyses of Full-Length Genome of FM DV OH99 Strain .VIROLOGICA SINICA, 2003, 18(3) : 259-264.

The Sequence Analyses of Full-Length Genome of FM DV OH99 Strain

  • Available online: 18 June 2003
  • The full-length genome of Foot-and-Mouth disease virus (FMDV) OH99strain was cloned by RT-PCR and 3’RACE, and sequenced. The result shows that the full-length genome of OH99 strain is 8040nt in length, including 5’NCR, Leader protein coding region, Polyprotein coding region, and 3’NCR Their nucleotide sequence is 1026nt, 603nt, 6318nt, and 93nt respectively. Besides, poly (A) tail was also coined by 3’RACE, it’s 56nt in length. The full-length genome of OH99 strain was compared with other reference strains, and its genome characteristic is also analyzed. With the help of some biological ware, The Phylogenetic tree obtained from the nucleotide sequence of VPlgene from OH99 strain and ten reference strains shows that OH99 strain could be ranked FMDV O style. Furthermore, this strain and OTY TW/97 are in the same genotype, their nucleotide sequence share high homology, but it shows remarkable divergence with other reference strains, including china99 strain. For example, two short genome segment was deleted in t

  • 加载中
  • 加载中

Article Metrics

Article views(4452) PDF downloads(887) Cited by()

Related
Proportional views

    The Sequence Analyses of Full-Length Genome of FM DV OH99 Strain

    • 1. Lanzhou Veterinary Research Institute,Lanzhou 730046,China

    Abstract: The full-length genome of Foot-and-Mouth disease virus (FMDV) OH99strain was cloned by RT-PCR and 3’RACE, and sequenced. The result shows that the full-length genome of OH99 strain is 8040nt in length, including 5’NCR, Leader protein coding region, Polyprotein coding region, and 3’NCR Their nucleotide sequence is 1026nt, 603nt, 6318nt, and 93nt respectively. Besides, poly (A) tail was also coined by 3’RACE, it’s 56nt in length. The full-length genome of OH99 strain was compared with other reference strains, and its genome characteristic is also analyzed. With the help of some biological ware, The Phylogenetic tree obtained from the nucleotide sequence of VPlgene from OH99 strain and ten reference strains shows that OH99 strain could be ranked FMDV O style. Furthermore, this strain and OTY TW/97 are in the same genotype, their nucleotide sequence share high homology, but it shows remarkable divergence with other reference strains, including china99 strain. For example, two short genome segment was deleted in t

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return