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Volume 18 Issue 4
August 2003
    Citation: XIE Yun—li, ZHANG Shu—yong, HUANG Ru, SHI Zheng—li*. A Modified Technique for Purifying White spot syndrome virus .VIROLOGICA SINICA, 2003, 18(4) : 391-393.
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    A Modified Technique for Purifying White spot syndrome virus

    • 1.

      Key laboratory of Molecular Virology,Joint·Laboratory of Invertebrate Hrology,Wuhan Institute of Hrology,Chinese Academy of Sciences，Wuhan 430071，China

    • Available online: 05 August 2003
    • Abstract：The envelope proteins of White spot syndrome virus(WSSV)are very fragile and easy to be destroyed during purification．It was difi cult to obtain a large quan tity of intact virions by routine sucrose gradient centrifugation．After modifying the sucrose gradient by adding citrate sodium ，we can obtain a large quantity of intact virions and nucleocapsids．Th is purified virions and nucleocapsids were subsequently used for an alyzing viral structural proteins an d DNA extraction．Th e result showed that this modified technique is very efi cient for virus purification．
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      A Modified Technique for Purifying White spot syndrome virus

      • 1. Key laboratory of Molecular Virology,Joint·Laboratory of Invertebrate Hrology,Wuhan Institute of Hrology,Chinese Academy of Sciences，Wuhan 430071，China

      Abstract: Abstract：The envelope proteins of White spot syndrome virus(WSSV)are very fragile and easy to be destroyed during purification．It was difi cult to obtain a large quan tity of intact virions by routine sucrose gradient centrifugation．After modifying the sucrose gradient by adding citrate sodium ，we can obtain a large quantity of intact virions and nucleocapsids．Th is purified virions and nucleocapsids were subsequently used for an alyzing viral structural proteins an d DNA extraction．Th e result showed that this modified technique is very efi cient for virus purification．

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