JIAO Cheng．Song, WANG Sheng．Qi, ZHANG He．Qiu, LING Shi．Gan.Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in M ammalian Cell .VIROLOGICA SINICA, 2003, 18(6): 538-540.
Citation:
JIAO Cheng．Song, WANG Sheng．Qi, ZHANG He．Qiu, LING Shi．Gan.
Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in M ammalian Cell .VIROLOGICA SINICA, 2003, 18(6)
: 538-540.
Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in M ammalian Cell
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1.
Department ofInfectious Diseases,Wuhan General Hospital,Guangzhou Military Command,Wuhan 430070,China
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2.
AcademyofMilitaryMedicalSciences，Beifing 100850,China
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Available online:
25 December 2003
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Abstract
An eukaryotic vector containing the full—length Hepatitis C virus(HCV)gene，pCi—HCV，was successfully constructed in this study．After~ansfected into HepG2 cells．it Was revealed to expleSS HCV C and NS3 proteins in the cloned cells by immunofluorescence and immunohistochemistry as Say respectively．It could be used for the experiments of the construction of HCV transgenic cellular model and the further study on the replication of HCV through cDNA．
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