Citation: OUYANG Zhi-quart, SUN Xiu-lian, DENG Fei, YUAN Li, HU Zhihong. Pathogenic and Immunohistochemical Studies of the HaSNPV Infected Helicoverpa armigera Larvae .VIROLOGICA SINICA, 2003, 18(6) : 603-606.

Pathogenic and Immunohistochemical Studies of the HaSNPV Infected Helicoverpa armigera Larvae

  • Available online: 25 December 2003
  • Abstract:Here we report the pathogensis process of Helicoverpa armigera larvae infected with Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)using H.E staining and immunohistoc hemistry with an tibody against polyhedrin.The fourth instar Helicoverpa armigera larvae were injected with 5×l PFU HaSNPV per larva.H.E staining showed that no apparent pathogensis could be detected in all tissues at 24 hours post infection(hpi).But at 48 hpi,the nucleus ofthe fat body an d trachea epidermis became swollen,an d the shape ofthe cells in these tissues were chan g~ .At 72 h P i,significant hispathogensis were observed in fat body,trachea epidermis an d the epidermis,while no chan ges were found in midgut an d muscles.At 96 hpi,the constitution of the fat body,trachea and epidermis were destroyed.Th e immunohistoc hemistry results indicated the po lyhedrin protein could not be detected at 24 hpi.At 48 hpi,it was found partially expressed in the tissues of fat body,trachea epidermis an d epidermis.From 72 to 96 hpi,the po lyhedrin was found heavily expressed in fat body, trachea epidermis and epidermis.No po lyhedrin was found expressed in midgut untill larvae dead an d themusculewasnotbe infected allthetime.

  • 加载中
  • 加载中

Article Metrics

Article views(4213) PDF downloads(630) Cited by()

Related
Proportional views

    Pathogenic and Immunohistochemical Studies of the HaSNPV Infected Helicoverpa armigera Larvae

    • 1. Key lab ofMolecular Virology and Joint-laboratory ofInvertebrate Virology,Wuhan Institute of Virology,Chinese Academyofsciences,Wuhan 430071,China

    Abstract: Abstract:Here we report the pathogensis process of Helicoverpa armigera larvae infected with Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)using H.E staining and immunohistoc hemistry with an tibody against polyhedrin.The fourth instar Helicoverpa armigera larvae were injected with 5×l PFU HaSNPV per larva.H.E staining showed that no apparent pathogensis could be detected in all tissues at 24 hours post infection(hpi).But at 48 hpi,the nucleus ofthe fat body an d trachea epidermis became swollen,an d the shape ofthe cells in these tissues were chan g~ .At 72 h P i,significant hispathogensis were observed in fat body,trachea epidermis an d the epidermis,while no chan ges were found in midgut an d muscles.At 96 hpi,the constitution of the fat body,trachea and epidermis were destroyed.Th e immunohistoc hemistry results indicated the po lyhedrin protein could not be detected at 24 hpi.At 48 hpi,it was found partially expressed in the tissues of fat body,trachea epidermis an d epidermis.From 72 to 96 hpi,the po lyhedrin was found heavily expressed in fat body, trachea epidermis and epidermis.No po lyhedrin was found expressed in midgut untill larvae dead an d themusculewasnotbe infected allthetime.

    Relative (13)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return