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Volume 19 Issue 1
February 2004
    Citation: ZHENG Lan-yan, LUO En-jie, LI Xin-ming, MOU Ling, ZHANG Xiao-tang, LU Run-ming. Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif .VIROLOGICA SINICA, 2004, 19(1) : 73-75.
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    Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif

    • 1.

      1. Department of Microbiology & Parasitology, China Medical University, Shenyang 110001,China

    • 2.

      The Municipal Hospital of Infectious Disease, Shenyang 110003, China

    • Corresponding author: LUO En-jie, 
    • Available online: 20 February 2004
    • To improve the effect of the gene immunization against Hantaan virus, we constructed the eukaryotic expression vector pTARGET-hans(ISS) containing Hantaan Virus S gene coding region and CpG motif by cloning S gene segment with CpG motif into eukaryotic expression vector pTARGETTM. After conformed by enzyme analysis, the recombinant expression vector pTARGET-hans(ISS) was transferred into Vero-E6 cells by electroporation and the transient expression of Hantaan virus nucleocapsid protein was detected by indirect immunofluorescence assay(IFA). In some transferred Vero-E6, the green fluorescence was showed, thus we can conclude that the eukaryotic expression vector pTARGET-hans(ISS) was successfully constructed and expressed in vitro, which will lay a foundation for further animal vaccination.
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      Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif

        Corresponding author: LUO En-jie,
      • 1. 1. Department of Microbiology & Parasitology, China Medical University, Shenyang 110001,China
      • 2. The Municipal Hospital of Infectious Disease, Shenyang 110003, China

      Abstract: To improve the effect of the gene immunization against Hantaan virus, we constructed the eukaryotic expression vector pTARGET-hans(ISS) containing Hantaan Virus S gene coding region and CpG motif by cloning S gene segment with CpG motif into eukaryotic expression vector pTARGETTM. After conformed by enzyme analysis, the recombinant expression vector pTARGET-hans(ISS) was transferred into Vero-E6 cells by electroporation and the transient expression of Hantaan virus nucleocapsid protein was detected by indirect immunofluorescence assay(IFA). In some transferred Vero-E6, the green fluorescence was showed, thus we can conclude that the eukaryotic expression vector pTARGET-hans(ISS) was successfully constructed and expressed in vitro, which will lay a foundation for further animal vaccination.

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