Study on Inhibition of TGEV Replication by Antisense RNA

In the study, we designed an antisense RNA targeting at the highly conserved sequence （26880-27184nt）of Transmissible gastroenteritis virus of swine,（TGEV）. The fragment cloned by PCR was recombined with retrovirus expression vector, pLXSN, and transfected into packaging cell line PA317 with Lipofectamine. The viral supenatants of the clones selected with G418 (500 g/mL) were detected by murine fibroblast NIH3T3. The highest viral titer pseudovirus was used to infect IBRS2 cell. PCR and RT-PCR analysis indicated that the pLXSN-N5’ had inserted into the genome of IBRS2 cell. The examination of cytopathic effect (CPE) showed that the antisense RNA could inhibit TGEV replication effectively.