Citation: HOU Wei, YANG Zhan-qiu, ZHU Run-qing, WEI Wen-jing, ZHOU Jun, ZHONG Qiong, WANG Wei, TANG Zhi-jiao, TANG Hong-bin, XIAN Qiao-yang, WANG Yong, WEN Li. Establishment of Pathogenicity Model of Severe Acute Respiratory Syndrome virus Infection on Macaca Rhesus .VIROLOGICA SINICA, 2004, 19(6) : 545-548.

Establishment of Pathogenicity Model of Severe Acute Respiratory Syndrome virus Infection on Macaca Rhesus

  • Corresponding author: YANG Zhan-qiu, 
  • Available online: 20 December 2004
  • To create the pathogenicity model of severe acute respiratory syndrome (SARS) on macaca rhesus, the macacu rhesus was infected with SARS coronavirus (CoV) was detected in different samples coming from macaca rhesue in different days by virus isolation, immunoflurescence assay, pathological inspection and reverse transcription polymerase chain reaction (RT-PCR). The results showed that SARS CoV were isolated from the above samples, and SARS CoV RNA could be detected in blood of the 2nd and 5th day, secreta of nose-throat of the 7th and 9th day, faeces of the 3th day, and faeces and urines of the 5th day after infection. Under the microscope, it was found in the group infected by SARS CoV that pulmonary alveolus interval had been broadened and had many lymphocytes and monocytes infiltrated. There was much exudation in the chamber pulmonary alveolus, which even formed the hyaline membrane. The organized pneumonia could be found in several pulmonary alveolus. great necrosis foci was also observed in the liver, companied by some inflammatory cells infiltrated. It may be successful to create the model of SARS on macaca rhesus, and show that the animal model can be used to evaluate anti-SARS CoV drugs and vaccines.

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    Establishment of Pathogenicity Model of Severe Acute Respiratory Syndrome virus Infection on Macaca Rhesus

      Corresponding author: YANG Zhan-qiu,
    • 1. 1. kry Laboratory of Virology of Ministery of Education, InstituIon of Virology, Medical School of Wuhan University, Wuhan 430071, China
    • 2. Department of Pathology, medical School of Wuhan University, Wuhan 430071, China
    • 3. Centre of Experimental Animal, Wuhan University, Wuhan 430071, China

    Abstract: To create the pathogenicity model of severe acute respiratory syndrome (SARS) on macaca rhesus, the macacu rhesus was infected with SARS coronavirus (CoV) was detected in different samples coming from macaca rhesue in different days by virus isolation, immunoflurescence assay, pathological inspection and reverse transcription polymerase chain reaction (RT-PCR). The results showed that SARS CoV were isolated from the above samples, and SARS CoV RNA could be detected in blood of the 2nd and 5th day, secreta of nose-throat of the 7th and 9th day, faeces of the 3th day, and faeces and urines of the 5th day after infection. Under the microscope, it was found in the group infected by SARS CoV that pulmonary alveolus interval had been broadened and had many lymphocytes and monocytes infiltrated. There was much exudation in the chamber pulmonary alveolus, which even formed the hyaline membrane. The organized pneumonia could be found in several pulmonary alveolus. great necrosis foci was also observed in the liver, companied by some inflammatory cells infiltrated. It may be successful to create the model of SARS on macaca rhesus, and show that the animal model can be used to evaluate anti-SARS CoV drugs and vaccines.

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