Citation: MA Wen-qiang, SHI Zheng-li. Expression and Distribution of White Spot Syndrome Virus ORF220 Gene in Insect Cells .VIROLOGICA SINICA, 2004, 19(6) : 572-575.

Expression and Distribution of White Spot Syndrome Virus ORF220 Gene in Insect Cells

  • Corresponding author: SHI Zheng-li, 
  • Available online: 20 December 2004
  • White spot syndrome virus ORF220 encodes a protein homologous to the gp130 receptors of eukaryote. prcvious studies showed that this protein was a minor structural protein. In this paper, the ORF220 was cloned into the pFastBacI fused with EGFP gene and co-transfected with AcBacmid into DH10B cells. The recombinant Bacmid containing fused ORF220 and EGFP genes was confirmed by PCR and then transfected into Sf21 cells. The green fluorencence was observed 3-5 d post-transfection under the fluorescent microscopy and this confirmed that the fusion protein was expressed in insect cells. The supernatant of the transfected cells was used to infect the Sf21 cells and the time course observation showed that the ORF220 product localized in both the cytoplasm and nucleus with no predilection.

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    Expression and Distribution of White Spot Syndrome Virus ORF220 Gene in Insect Cells

      Corresponding author: SHI Zheng-li,
    • 1. 1. Key Laboratory of Molecular Virology, Joint-Laboratory of Invertebrate Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071,China
    • 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China

    Abstract: White spot syndrome virus ORF220 encodes a protein homologous to the gp130 receptors of eukaryote. prcvious studies showed that this protein was a minor structural protein. In this paper, the ORF220 was cloned into the pFastBacI fused with EGFP gene and co-transfected with AcBacmid into DH10B cells. The recombinant Bacmid containing fused ORF220 and EGFP genes was confirmed by PCR and then transfected into Sf21 cells. The green fluorencence was observed 3-5 d post-transfection under the fluorescent microscopy and this confirmed that the fusion protein was expressed in insect cells. The supernatant of the transfected cells was used to infect the Sf21 cells and the time course observation showed that the ORF220 product localized in both the cytoplasm and nucleus with no predilection.

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