Citation: WANG Fu-xiang, SUN Yong-tao, PAN Yan. SDF-1 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine .VIROLOGICA SINICA, 2005, 20(3) : 243-246.

SDF-1 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine

  • Available online: 20 June 2005
  • To investigate the effect of SDF-1 gene immunization on immune response induced by HIV-1(Human immunodeficiency virus) nucleic acid vaccine and to explore new strategies for therapeutic HIV DNA vaccine. Balb/c mice were immunized with pCI-neoGAG alone or co-administered with the DNA encoding for SDF-1.Their sera were collected for analyzing anti-HIV antibody and IFN-γ by ELISA , and splenocytes were isolated for detecting antigen-specific lymphoproliferative responses and specific CTL response by MTT assay and LDH assay ,respectively. Our result showed that the anti-HIV antibody titers of mice co-immunized with pCI-neoGAG and the DNA encoding for SDF-1 were lower than that of mice immunized with pCI-neoGAG alone(P0.01).The IFN-γ level of mice co-immunized with pCI-neoGAG and the DNA encoding for SDF-1was higher than that of mice immunized with pCI-neoGAG alone(P0.01).Furthermore, compared with mice injected with pCI-neoGAG alone, the specific CTL cytotoxity activity and antigen-specific lymphoproliferative

  • 加载中
  • 加载中

Article Metrics

Article views(3775) PDF downloads(932) Cited by()

Related
Proportional views

    SDF-1 Gene Modulates Immune Responses Induced by HIV-1 Nucleic Acid Vaccine

    • 1. 

    Abstract: To investigate the effect of SDF-1 gene immunization on immune response induced by HIV-1(Human immunodeficiency virus) nucleic acid vaccine and to explore new strategies for therapeutic HIV DNA vaccine. Balb/c mice were immunized with pCI-neoGAG alone or co-administered with the DNA encoding for SDF-1.Their sera were collected for analyzing anti-HIV antibody and IFN-γ by ELISA , and splenocytes were isolated for detecting antigen-specific lymphoproliferative responses and specific CTL response by MTT assay and LDH assay ,respectively. Our result showed that the anti-HIV antibody titers of mice co-immunized with pCI-neoGAG and the DNA encoding for SDF-1 were lower than that of mice immunized with pCI-neoGAG alone(P0.01).The IFN-γ level of mice co-immunized with pCI-neoGAG and the DNA encoding for SDF-1was higher than that of mice immunized with pCI-neoGAG alone(P0.01).Furthermore, compared with mice injected with pCI-neoGAG alone, the specific CTL cytotoxity activity and antigen-specific lymphoproliferative

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return