Citation: MA Xin-yan, GU Qin-sheng, LIU Li-feng, LI Ning, YANG Min-he, PENG Bin, LI Li. Cloning and Construction of Plant Expression of the Coat Protein Gene of a Melon Isolate of Cucumber Mosaic Virus .VIROLOGICA SINICA, 2005, 20(3) : 307-310.

Cloning and Construction of Plant Expression of the Coat Protein Gene of a Melon Isolate of Cucumber Mosaic Virus

  • Available online: 20 June 2005
  • The coat protein gene of Cucumber mosaic virus (CMV) from melon isolate (CH99/ 90) was amplified by RT2PCR from the total RNA of infected zucchini leaves and cloned into pUCm2 T vector . The gene consisted of 657 nucleotides encoding 218 putative amino acids. Nucleotide sequence alignments showed that the CP gene shared 92. 2 %~ 93. 9 % homology wit h that of CMV subgroup I strains , whereas only 76. 8 %~77. 8 % homology with that of CMV subgroup II strains. The nucleotide sequence shared 91. 8 %~93. 4 % homology with CMV isolate previously reported from China except for XB isolate. This melon isolate was classified into subgroup I based on these data. The gene was constructed into plant expression vector by using intermediate vector pJIT163 ( recombinant plasmid designated as pBCP) . The recombinant plasmid was transferred into competent cells of Agrobacterium tume faciens LBA4404. Transformation of the gene into watermelon is undertaking.

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    Cloning and Construction of Plant Expression of the Coat Protein Gene of a Melon Isolate of Cucumber Mosaic Virus

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    Abstract: The coat protein gene of Cucumber mosaic virus (CMV) from melon isolate (CH99/ 90) was amplified by RT2PCR from the total RNA of infected zucchini leaves and cloned into pUCm2 T vector . The gene consisted of 657 nucleotides encoding 218 putative amino acids. Nucleotide sequence alignments showed that the CP gene shared 92. 2 %~ 93. 9 % homology wit h that of CMV subgroup I strains , whereas only 76. 8 %~77. 8 % homology with that of CMV subgroup II strains. The nucleotide sequence shared 91. 8 %~93. 4 % homology with CMV isolate previously reported from China except for XB isolate. This melon isolate was classified into subgroup I based on these data. The gene was constructed into plant expression vector by using intermediate vector pJIT163 ( recombinant plasmid designated as pBCP) . The recombinant plasmid was transferred into competent cells of Agrobacterium tume faciens LBA4404. Transformation of the gene into watermelon is undertaking.

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