Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene
Abstract: A Plasmid DNA vaccine pcDNA-gB expressing gB protein of infectious laryngotracheitis virus(ILTV) Yantai strain was constructed. The expression of viral protein from constructed DNA vaccine was confirmed by an in vitro transcription /translation system and transtection in 293-T cells. To evaluate the prophylactic efficacy of the DNA vaccine, 4-week-old chickens were inoculated with pcDNA-gB plasmid and rFPV- gB-gD-IgY, respectively followed by a booster of rFPV-gB-gD-IgY and pcDNA-gB. Anti-ILTV antibodies and T cell proliferation were detected. The results indicated that both vaccines could elicit specific humeral immune and cellular immune responses. Compared with attenuated vaccines, rFPV-gB-gD-IgY/pcDNA-gB elicited not only similar antibody level of ILTV but stronger T cell proliferation．The study lays foundation on further investigation for the new type of vaccines against ILTV.