VP1 Sequence Analysis and Serological Identification of a New Isolated Enterovirus ECHO20
Abstract: Hepatitis A virus and ECHO20 viruses were isolated from faeces of hepatitis A patients in Luxi County, Yunnan Province. The combined and single serological neutralization antibody tests of enterovirus demonstrated that the patients were infected by both viruses. One strain, named LX0901, was selected for RT-PCR by using the general primers of VP1 of enterovirus. The product was purified and sequenced. The data were deposited in GenBank and compared with the complete VP1 sequence of 3 isolated ECHO20 strains. Analysis indicated that the LX0901 strain was ECHO20 virus, which was in agreement with serological neutralization tests. The variations of amino acids were not so high with an identity of more than 97%, but the variation were distributed throughout the genome.