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Volume 22 Issue 6
December 2007
    Citation: Ming-hua LI, Shao-you LI, Hou-jun XIA, Lu WANG, Yuan-yuan WANG, Gao-hong ZHANG, Yong-tang ZHENG. Establishment of AIDS Animal Model with SIVmac239 Infected Chinese Rhesus Monkey .VIROLOGICA SINICA, 2007, 22(6) : 509-516.
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    Establishment of AIDS Animal Model with SIVmac239 Infected Chinese Rhesus Monkey

    • 1.

      Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China

    • 2.

      Graduate School of the Chinese Academy of Sciences, Beijing 100049, China

    • Corresponding author: Yong-tang ZHENG, zhengyt@mail.kiz.ac.cn
    • These authors contributed equally to this work
    • Received Date: 03 July 2007
      Accepted Date: 16 October 2007
      Available online: 01 December 2007

      Fund Project: Key Scientific and Technological projects of China 2004BA719A14Yunnan province 2004NG12National 973 project of China 2006CB504300Yunnan province 2006PT08National 973 project of China 2006CB504200The Knowledge Innovation Program KSCX1-YW-R-15The Natural Science Foundation of China 30671960The Natural Science Foundation of China 30471605

    • In the present research, two Chinese rhesus monkeys were inoculated intravenously with 5000 TCID50 of SIVmac239. The changes in the numbers of CD4+ T lymphocyte in peripheral blood, plasma viral loads, proviral DNA and humoral antibodies against virus were periodically monitored during 121 days. At the early stage of infection, proviral DNA had been detected in PBMCs, and infectious SIVmac239 virus had been isolated from PBMCs. At the same period, the numbers of CD4+ T lymphocytes were significantly decreased, and maintained at low level during the 121-day period of infection. Plasma viral loads reached the peak at week 2 post-inoculation and kept at a steady state subsequently. Moreover, antibodies against viral proteins were detected from plasma. All the results showed that the two Chinese rhesus monkeys had been infected with SIVmac239 successfully. This animal model can be applied for further AIDS researches.
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      1. Abel K, Compton L, Rourke T, et al. 2003. Simian-Human immunodeficiency virus SHIV89.6-induced pro-tection against intravaginal challenge with pathogenic SIVmac239 is independent of the route of immunization and is associated with a combination of cytotoxic T-lymphocyte and alpha interferon responses. J Virol, 77 (5): 3099-3118.
          doi: 10.1128/JVI.77.5.3099-3118.2003

      2. Cong Z, Tu X M, Jiang H, et al.2005. Application of PCR techniques in detection of simian immunodeficiency virus (SIV) in monkey infection models.Acta Lab Anim Sci Sin, 13 (2): 84-87. (in Chinese).

      3. Estaquier J, Monceaux V, Cumont M C, et al. 2000. Early changes in peripheral blood T cells during primary infection of rhesus macaques with a pathogenic SIV. J Med Primatol, 29 (3-4): 127-135.

      4. Girard M, Mahoney J, Wei Q, et al. 1998. Genital infection of female chimpanzees with human immun-odeficiency virus type 1. AIDS Res Hum Retrovirused, 14 (15): 1357-1367.
          doi: 10.1089/aid.1998.14.1357

      5. Guo W Z, Lu Y Z, Wu X X, et al. 2005. Experimental study on ATA prescription in treating monkey with chronic infection of simian immunodeficiency virus. J Guangzhou Univ Tradit Chin Medi, 22 (4): 296-298 (in Chinese).

      6. Kuwata T, Kodama M, Sato A, et al. 2007. Contribution of monocytes to viral replication in macaques during acute infection with simian immunodeficiency virus. AIDS Res Hum Retroviruses, 23 (3): 372-380.
          doi: 10.1089/aid.2006.0208

      7. Li M H, He Z Y, Zheng Y T. 2005. The scapegoat of human AIDS research: non-human primate models. Chin J Nat, 27 (4): 208-212. (in Chinese).

      8. Li M H, Zhang G H, Sun T, et al. 2007. The value of nonhuman primate animal models in anti-HIV drug studies.Chin J New Drug, 16: (16): 1237-1242. (in Chinese).

      9. Ling B, Veazey R S, Penedo C, et al. 2002. Longitudinal follow up of SIVmac phthogenesis in rhesus macaques of Chinese origin: emergence of B cell lymphoma. J Med Primatol, 31: 154-163.
          doi: 10.1034/j.1600-0684.2002.02001.x

      10. Lu Y Z, Wu X X, Tu X M, et al. 1994. The establishment of SIV-infected macaque models [J]. Acta Lab Anim Sci Sin, 2 (2): 94-101. (in Chinese).

      11. McDermott A B, Mitchen J, Piaskowski S, et al. 2004. Repeated low-dose mucosal simian immunodeficiency virus SIVmac239 challenge results in the same viral and immunological kinetics as high-dose challenge: a model for the evaluation of vaccine efficacy in nonhuman primates. J Virol, 78 (6): 3140-3144.
          doi: 10.1128/JVI.78.6.3140-3144.2004

      12. Metzner K J, Moretto W J, Donahoe S M, et al.2005. Evaluation of CD8+ T-cell and antibody responses follo-wing transient increased viraemia in rhesus macaques infected with live, attenuated simian immunodeficiency virus.J Gen Virol, 86: 3375-3384.
          doi: 10.1099/vir.0.81206-0

      13. Miller C J, Abel K. 2005. Immune mechanisms associated with protection from vaginal SIV challenge in rhesus monkeys infected with virulence-attenuated SHIV89.6. J Med Primatol, 34 (5-6): 271-281.
          doi: 10.1111/jmp.2005.34.issue-5-6

      14. Muller M C, Barre-Sinoussi F. 2003. SIVagm: genetic and biological features associated with replication. Front Biosci, 8: 1170-1185.
          doi: 10.2741/1130

      15. Yamamoto H, Kawada M, Tsukamoto T, et al.2007. Vaccine-based, long-term, stable control of simian/human immunodeficiency virus 89.6PD replication in rhesus macaques. J Gen Virol, 88: 652-659.
          doi: 10.1099/vir.0.82469-0

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      Establishment of AIDS Animal Model with SIVmac239 Infected Chinese Rhesus Monkey

        Corresponding author: Yong-tang ZHENG, zhengyt@mail.kiz.ac.cn
      • 1. Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
      • 2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
      • Received Date: 03 July 2007
      • Accepted Date: 16 October 2007
      Fund Project:  Key Scientific and Technological projects of China 2004BA719A14Yunnan province 2004NG12National 973 project of China 2006CB504300Yunnan province 2006PT08National 973 project of China 2006CB504200The Knowledge Innovation Program KSCX1-YW-R-15The Natural Science Foundation of China 30671960The Natural Science Foundation of China 30471605

      Abstract: In the present research, two Chinese rhesus monkeys were inoculated intravenously with 5000 TCID50 of SIVmac239. The changes in the numbers of CD4+ T lymphocyte in peripheral blood, plasma viral loads, proviral DNA and humoral antibodies against virus were periodically monitored during 121 days. At the early stage of infection, proviral DNA had been detected in PBMCs, and infectious SIVmac239 virus had been isolated from PBMCs. At the same period, the numbers of CD4+ T lymphocytes were significantly decreased, and maintained at low level during the 121-day period of infection. Plasma viral loads reached the peak at week 2 post-inoculation and kept at a steady state subsequently. Moreover, antibodies against viral proteins were detected from plasma. All the results showed that the two Chinese rhesus monkeys had been infected with SIVmac239 successfully. This animal model can be applied for further AIDS researches.

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