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Volume 25 Issue 2
April 2010
    Citation: Qing-chan ZHANG, Dong-min ZHAO, Hui-jun GUO, Zhi-zhong CUI. Isolation and Identification of a Subgroup A Avian Leukosis Virus from Imported Meat-type Grand-parent Chickens* .VIROLOGICA SINICA, 2010, 25(2) : 130-136.  http://dx.doi.org/10.1007/s12250-010-3095-3
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    Isolation and Identification of a Subgroup A Avian Leukosis Virus from Imported Meat-type Grand-parent Chickens*

    cstr: 32224.14.s12250-010-3095-3

    • College of Veterinary Medicine Shandong Agricultural University, Taian 271018, China

    • Corresponding author: Zhi-zhong CUI, zzcui@sdau.edu.cn
    • Received Date: 21 August 2009
      Accepted Date: 26 November 2009
      Available online: 01 April 2010

      Fund Project: This work was supported by a grant from Shandong province and a grant #200803019

    • An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that SDAU09C1 fell into subgroup A; it had homology of 88.8% - 90.3% to 6 reference strains of subgroup A, much higher compared to other subgroups including subgroup B. This is the first report for ALV of subgroup A isolated from imported breeders.
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      1. Cheng Z, Zhang L, Liu S, et al. 2005. Emerging of Avian leukosis virus subgroup J in a flock of Chinese local breed. Acta Microbiologica Sinica, 45:584-587. (in Chinese)

      2. Cui Z, Du Y, Zhang Z, et al.2003. Comparison of Chinese field strains of avian leukosis subgroup J viruses with prototype strain HPRS-103 and United States strains.Avian Dis, 47:1321-1330.
          doi: 10.1637/6085

      3. Du Y, Cui Z, Qin A, et al.2000. Isolation of subgroup J avian leukosis viruses and their partial sequence comparison (in Chinese with English abstract).Chin J Virol. 16: 341-346. (in Chinese)

      4. Fadly A, Silva R, Hunt H, et al. 2006. Isolation and characterization of an adventitious avian leukosis virus isolated from commercial Marek's disease vaccines. Avian Dis, 50 (3):380-385.
          doi: 10.1637/7497-122905R.1

      5. Fadly A M, Nair V. 2008. Leukosis/Sarcoma Group. In: Diseases of Poultry (Saif Y M, Fadly A M, Glisson J R, et al. ed.). 12th ed. Ames: Blackwell Publishing. p514-568.

      6. Silva R F, Fadly A M, Taylor S P. 2007. Development of a Polymerase Chain Reaction of differentiate avain leukosis virus (ALV) subgroups:detection of an ALV contaminant in commercial Marek's Disease vaccines. Avian Dis, 51(3):663-667.
          doi: 10.1637/0005-2086(2007)51[663:DOAPCR]2.0.CO;2

      7. Sun S, Cui Z. 2007.Epidemiological and pathological studies of subgroup J avian leukosis virus infections in Chinese local"yellow" chickens.Avian Pathol, 36(3): 221-226.
          doi: 10.1080/03079450701332345

      8. Wang H, Cui Z. 2008.The identification and sequence analysis of ALV-J isolated from layers (In Chinese with English abstract).Chin J Virol, 24:369-375. (in Chinese)

      9. Xu B, Dong W, Yu C, et al. 2004.Occurrence of avian leukosis virus subgroup J in commercial layer flocks in Avian Pathol. 33(1): 13-17.
          doi: 10.1080/03079450310001636237a

      10. Zavala G, Cheng S.2006. Detection and characterization of avian leukosis virus in Marek's disease vaccines. Avain Dis, 50:209-215.
          doi: 10.1637/7444-092405R.1

      11. Zhang Z, Cui Z, Zhao H, et al.2002. Pathological study on subgroup J of avian leukosis and isolation of virus from commercial broiler flocks.J China Veter, 38(6):6-8. (in Chinese)

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      Isolation and Identification of a Subgroup A Avian Leukosis Virus from Imported Meat-type Grand-parent Chickens*

        Corresponding author: Zhi-zhong CUI, zzcui@sdau.edu.cn
      • College of Veterinary Medicine Shandong Agricultural University, Taian 271018, China
      • Received Date: 21 August 2009
      • Accepted Date: 26 November 2009
      Fund Project:  This work was supported by a grant from Shandong province and a grant #200803019

      Abstract: An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that SDAU09C1 fell into subgroup A; it had homology of 88.8% - 90.3% to 6 reference strains of subgroup A, much higher compared to other subgroups including subgroup B. This is the first report for ALV of subgroup A isolated from imported breeders.

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