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Volume 32 Issue 2
April 2017
    Citation: Lianghua Guo, Xiuguo Hua, Wen Zhang, Shixing Yang, Quan Shen, Haibing Hu, Jingjiao Li, Zhijian Liu, Xiaochun Wang, Hua Wang, Chenglin Zhou, Li Cui. Viral metagenomics analysis of feces from coronary heart disease patients reveals the genetic diversity of the Microviridae .VIROLOGICA SINICA, 2017, 32(2) : 130-138.  http://dx.doi.org/10.1007/s12250-016-3896-0
    shu

    Viral metagenomics analysis of feces from coronary heart disease patients reveals the genetic diversity of the Microviridae

    Virome and Microviridae genetic diversity of CHD patients

    cstr: 32224.14.s12250-016-3896-0
    • 1.

      School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

    • 2.

      School of Medicine, Jiangsu University, Zhenjiang 212013, China

    • 3.

      Department of Laboratory Medicine, Jiangsu Taizhou People's Hospital, Taizhou 225300, China

    • Corresponding author: Li Cui, lcui@sjtu.edu.cn
    • Lianghua Guo, ORCID: 0000-0002-7773-5035
    • Received Date: 21 October 2016
      Accepted Date: 22 March 2017
      Published Date: 17 April 2017
      Available online: 01 April 2017
    • Recent studies have declared that members of the ssDNA virus family Microviridae play an important role in multiple environments,as they have been found taking a dominant position in the human gut.The aim of this study was to analyze the overall composition of the gut virome in coronary heart disease (CHD) patients,and try to discover the potential link between the human gut virome and CHD.Viral metagenomics methods were performed to detect the viral sequences in fecal samples collected from CHD inpatients and healthy persons as controls.We present the analysis of the virome composition in these CHD patients and controls.Our data shows that the virome composition may be linked to daily living habits and the medical therapy of CHD. Virgaviridae and Microviridae were the two dominant types of viruses found in the enteric virome of CHD patients.Fourteen divergent viruses belonging to the family Microviridae were found,twelve of which were grouped into the subfamily Gokushovirinae,while the remaining two strains might represent two new subfamilies within Microviridae,according to the phylogenetic analysis.In addition,the genomic organization of these viruses has been characterized.
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      Viral metagenomics analysis of feces from coronary heart disease patients reveals the genetic diversity of the Microviridae

        Corresponding author: Li Cui, lcui@sjtu.edu.cn
      • Lianghua Guo, ORCID: 0000-0002-7773-5035
      • 1. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
      • 2. School of Medicine, Jiangsu University, Zhenjiang 212013, China
      • 3. Department of Laboratory Medicine, Jiangsu Taizhou People's Hospital, Taizhou 225300, China
      • Received Date: 21 October 2016
      • Accepted Date: 22 March 2017
      • Published Date: 17 April 2017

      Abstract: Recent studies have declared that members of the ssDNA virus family Microviridae play an important role in multiple environments,as they have been found taking a dominant position in the human gut.The aim of this study was to analyze the overall composition of the gut virome in coronary heart disease (CHD) patients,and try to discover the potential link between the human gut virome and CHD.Viral metagenomics methods were performed to detect the viral sequences in fecal samples collected from CHD inpatients and healthy persons as controls.We present the analysis of the virome composition in these CHD patients and controls.Our data shows that the virome composition may be linked to daily living habits and the medical therapy of CHD. Virgaviridae and Microviridae were the two dominant types of viruses found in the enteric virome of CHD patients.Fourteen divergent viruses belonging to the family Microviridae were found,twelve of which were grouped into the subfamily Gokushovirinae,while the remaining two strains might represent two new subfamilies within Microviridae,according to the phylogenetic analysis.In addition,the genomic organization of these viruses has been characterized.

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