Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2

Han Xiao; Hengrui Hu; Yijia Guo; Jiang Li; Le Wen; Wen-Bo Zeng; Manli Wang; Min-Hua Luo; Zhihong Hu

doi:10.1016/j.virs.2023.03.005

June 2023

Citation: Han Xiao, Hengrui Hu, Yijia Guo, Jiang Li, Le Wen, Wen-Bo Zeng, Manli Wang, Min-Hua Luo, Zhihong Hu. Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2 .VIROLOGICA SINICA, 2023, 38(3) : 373-379. http://dx.doi.org/10.1016/j.virs.2023.03.005

Construction and characterization of a synthesized herpes simplex virus H129-Syn-G2

Han Xiao ^a,b ,
Hengrui Hu ^a ,
Yijia Guo ^a,b ,
Jiang Li ^a ,
Le Wen ^a ,
Wen-Bo Zeng ^{a
,,} ,
Manli Wang ^{a,b
,,} ,
Min-Hua Luo ^{a,b
,,} ,
Zhihong Hu ^{a
,,}

a. State Key Laboratory of Virology, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China;
b. University of Chinese Academy of Sciences, Beijing, 100049, China

Corresponding author: Wen-Bo Zeng, zengwb@wh.iov.cn
Manli Wang, wangml@wh.iov.cn
Min-Hua Luo, luomh@wh.iov.cn
Zhihong Hu, huzh@wh.iov.cn
Received Date: 27 December 2022
Accepted Date: 15 March 2023
Available online: 20 March 2023

Abstract

Herpes simplex virus type 1 (HSV-1) causes lifelong infections worldwide, and currently there is no efficient cure or vaccine. HSV-1-derived tools, such as neuronal circuit tracers and oncolytic viruses, have been used extensively; however, further genetic engineering of HSV-1 is hindered by its complex genome structure. In the present study, we designed and constructed a synthetic platform for HSV-1 based on H129-G4. The complete genome was constructed from 10 fragments through 3 rounds of synthesis using transformation-associated recombination (TAR) in yeast, and was named H129-Syn-G2. The H129-Syn-G2 genome contained two copies of the gfp gene and was transfected into cells to rescue the virus. According to growth curve assay and electron microscopy results, the synthetic viruses exhibited more optimized growth properties and similar morphogenesis compared to the parental virus. This synthetic platform will facilitate further manipulation of the HSV-1 genome for the development of neuronal circuit tracers, oncolytic viruses, and vaccines.
- Herpes simplex virus type 1 (HSV-1)
- , Neuronal circuit tracers
- , H129-Syn-G2
- , H129-G4
- , Synthetic biology

Electronic Supplementary Material

10.1016j.virs.2023.03.005-ESM.docx
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