Prevalence, variation, and transmission patterns of human respiratory syncytial virus from pediatric patients in Hubei, China during 2020–2021

Yi Yan; Decheng Wang; Ying Li; Zhiyong Wu; Haizhou Liu; Yue Shi; Xiaoxia Lu; Di Liu

doi:10.1016/j.virs.2023.05.001

June 2023

Citation: Yi Yan, Decheng Wang, Ying Li, Zhiyong Wu, Haizhou Liu, Yue Shi, Xiaoxia Lu, Di Liu. Prevalence, variation, and transmission patterns of human respiratory syncytial virus from pediatric patients in Hubei, China during 2020–2021 .VIROLOGICA SINICA, 2023, 38(3) : 363-372. http://dx.doi.org/10.1016/j.virs.2023.05.001

Prevalence, variation, and transmission patterns of human respiratory syncytial virus from pediatric patients in Hubei, China during 2020–2021

Yi Yan ^a,b,c ,
Decheng Wang ^a,b,c ,
Ying Li ^a,b,c,d,e,f ,
Zhiyong Wu ^a,b,c,d ,
Haizhou Liu ^a,b,c ,
Yue Shi ^a,b,c ,
Xiaoxia Lu ^{e,f
,,} ,
Di Liu ^{a,b,c,d
,,}

a. CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China;
b. National Virus Resource Center, Chinese Academy of Sciences, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Wuhan, 430071, China;
c. Computational Virology Group, Center for Bacteria and Viruses Resources and Bioinformation, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China;
d. University of Chinese Academy of Sciences, Beijing, 101408, China;
e. Department of Respiratory Medicine, Wuhan Children' Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China;
f. Pediatric Respiratory Disease Laboratory, Institute of Maternal and Child Health, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China

Corresponding author: Xiaoxia Lu, lusi74@163.com
Di Liu, liud@wh.iov.cn
Received Date: 17 February 2022
Accepted Date: 26 April 2023
Available online: 03 May 2023

Abstract

Human respiratory syncytial virus (RSV) is a severe threat to children and a main cause of acute lower respiratory tract infections. Nevertheless, the intra-host evolution and inter-regional diffusion of RSV are little known. In this study, we performed a systematic surveillance in hospitalized children in Hubei during 2020–2021, in which 106 RSV-positive samples were detected both clinically and by metagenomic next generation sequencing (mNGS). RSV-A and RSV-B groups co-circulated during surveillance with RSV-B being predominant. About 46 high-quality genomes were used for further analyses. A total of 163 intra-host nucleotide variation (iSNV) sites distributed in 34 samples were detected, and glycoprotein (G) gene was the most enriched gene for iSNVs, with non-synonymous substitutions more than synonymous substitutions. Evolutionary dynamic analysis showed that the evolutionary rates of G and NS2 genes were higher, and the population size of RSV groups changed over time. We also found evidences of inter-regional diffusion from Europe and Oceania to Hubei for RSV-A and RSV-B, respectively. This study highlighted the intra-host and inter-host evolution of RSV, and provided some evidences for understanding the evolution of RSV.
- Respiratory syncytial virus (RSV)
- , Intra-host single nucleotide variation (iSNV)
- , Evolutionary dynamic
- , Inter-regional diffusion

Electronic Supplementary Material

10.1016j.virs.2023.05.001-ESM.docx
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