Citation: Xiaoli Wang, Yu He, Jiaqi Guo, Zhen Wu, Andres Merits, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Qun Gao, Di Sun, Anchun Cheng, Shun Chen. Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice .VIROLOGICA SINICA, 2023, 38(5) : 827-831.  http://dx.doi.org/10.1016/j.virs.2023.07.006

Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice

  • Highlights:1. TMUV can infect Kunming mice by intracranial injection, and the pathogenicity of mosquito TMUV is higher than that of duck TMUV.2. TMUV can infect SPF ducklings by intramuscular injection, and the pathogenicity of duck TMUV is higher than that of mosquito TMUV.3. SPF ducklings infected with mosquito TMUV did not produce viremia at 3, 5 and 7 dpi.

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    2. Guo, J., He, Y., Wang, X., Jiang, B., Lin, X., Wang, M., Jia, R., Zhu, D., Liu, M., Zhao, X., Yang, Q., Wu, Y., Chen, S., Cheng, A., 2020. Stabilization of a full-length infectious cDNA clone for duck Tembusu virus by insertion of an intron. J Virol Methods 283, 113922.

    3. Kimura, T., Sasaki, M., Okumura, M., Kim, E., Sawa, H., 2010. Flavivirus encephalitis:pathological aspects of mouse and other animal models. Vet Pathol 47, 806-818.

    4. Mao, L., He, Y., Wu, Z., Wang, X., Guo, J., Zhang, S., Wang, M., Jia, R., Zhu, D., Liu, M., Zhao, X., Yang, Q., Mao, S., Wu, Y., Zhang, S., Huang, J., Ou, X., Gao, Q., Sun, D., Cheng, A., Chen, S., 2022. Stem-Loop I of the Tembusu Virus 3'-Untranslated Region Is Responsible for Viral Host-Specific Adaptation and the Pathogenicity of the Virus in Mice. Microbiology spectrum 10, e0244922.

    5. Platt, G.S., Way, H.J., Bowen, E.T., Simpson, D.I., Hill, M.N., Kamath, S., Bendell, P.J., Heathcote, O.H., 1975. Arbovirus infections in Sarawak, October 1968——February 1970 Tembusu and Sindbis virus isolations from mosquitoes. Ann Trop Med Parasitol 69, 65-71.

    6. Prestwood, T.R., May, M.M., Plummer, E.M., Morar, M.M., Yauch, L.E., Shresta, S., 2012. Trafficking and replication patterns reveal splenic macrophages as major targets of dengue virus in mice. J Virol 86, 12138-12147.

    7. Stonedahl, S., Clarke, P., Tyler, K.L., 2020. The Role of Microglia during West Nile Virus Infection of the Central Nervous System. Vaccines (Basel) 8, 485.

    8. Su, J., Li, S., Hu, X., Yu, X., Wang, Y., Liu, P., Lu, X., Zhang, G., Hu, X., Liu, D., Li, X., Su, W., Lu, H., Mok, N.S., Wang, P., Wang, M., Tian, K., Gao, G.F., 2011. Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus. PLoS One 6, e18106.

    9. Sun, X., Liu, E., Iqbal, A., Wang, T., Wang, X., Haseeb, A., Ahmed, N., Yang, P., Chen, Q., 2019. The dynamic distribution of duck Tembusu virus in the spleen of infected shelducks. BMC Vet Res 15, 112.

    10. Ti, J., Zhang, M., Li, Z., Li, X., Diao, Y., 2016. Duck Tembusu Virus Exhibits Pathogenicity to Kunming Mice by Intracerebral Inoculation. Front Microbiol 7, 190.

    11. Wang, X., He, Y., Guo, J., Jiang, B., Wang, M., Jia, R., Zhu, D., Liu, M., Zhao, X., Yang, Q., Wu, Y., Zhang, S., Liu, Y., Zhang, L., Yu, Y., Cheng, A., Chen, S., 2021. Construction of an Infectious Clone for Mosquito-Derived Tembusu Virus Prototypical Strain. Virol Sin 36, 1678-1681.

    12. Yan, D., Shi, Y., Wang, H., Li, G., Li, X., Wang, B., Su, X., Wang, J., Teng, Q., Yang, J., Chen, H., Liu, Q., Ma, W., Li, Z., 2018. A Single Mutation at Position 156 in the Envelope Protein of Tembusu Virus Is Responsible for Virus Tissue Tropism and Transmissibility in Ducks. J Virol 92, e00427-18.

    13. Yang, S., Huang, Y., Shi, Y., Bai, X., Yang, P., Chen, Q., 2021. Tembusu Virus entering the central nervous system caused nonsuppurative encephalitis without disrupting the blood-brain barrier. J Virol 95, e02191-20.

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    15. Zhu, K., Huang, J., Jia, R., Zhang, B., Wang, M., Zhu, D., Chen, S., Liu, M., Yin, Z., Cheng, A., 2015. Identification and molecular characterization of a novel duck Tembusu virus isolate from Southwest China. Arch Virol 160, 2781-2790.

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    Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice

      Corresponding author: Anchun Cheng, chenganchun@vip.163.com
      Corresponding author: Shun Chen, shunchen@sicau.edu.cn
    • a. Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China;
    • b. Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China;
    • c. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China;
    • d. Institute of Technology, University of Tartu, Tartu, 999148, Estonia

    Abstract: Highlights:1. TMUV can infect Kunming mice by intracranial injection, and the pathogenicity of mosquito TMUV is higher than that of duck TMUV.2. TMUV can infect SPF ducklings by intramuscular injection, and the pathogenicity of duck TMUV is higher than that of mosquito TMUV.3. SPF ducklings infected with mosquito TMUV did not produce viremia at 3, 5 and 7 dpi.

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