Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice

Xiaohui Zan; Shirong Wang; Tianqi Zhang; Ying Li; Chunge Zhang; Cun Fu; Jialei Wang; Youzhi Wu; Yanhua Ma; Zhifei Li; Yan Wang; Hao Wang; Yuhai Bi; Wei Wang

doi:10.1016/j.virs.2023.09.006

December 2023

Citation: Xiaohui Zan, Shirong Wang, Tianqi Zhang, Ying Li, Chunge Zhang, Cun Fu, Jialei Wang, Youzhi Wu, Yanhua Ma, Zhifei Li, Yan Wang, Hao Wang, Yuhai Bi, Wei Wang. Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice .VIROLOGICA SINICA, 2023, 38(6) : 966-970. http://dx.doi.org/10.1016/j.virs.2023.09.006

Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice

Xiaohui Zan ^a ,
Shirong Wang ^a ,
Tianqi Zhang ^a ,
Ying Li ^a ,
Chunge Zhang ^b,d ,
Cun Fu ^a ,
Jialei Wang ^a ,
Youzhi Wu ^a ,
Yanhua Ma ^a,e ,
Zhifei Li ^a ,
Yan Wang ^a ,
Hao Wang ^a ,
Yuhai Bi ^{b,d
,,} ,
Wei Wang ^{a,c
,,}

a. State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, 010070, China;
b. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), CAS-TWAS Center of Excellence for Emerging Infectious Diseases (CEEID), Chinese Academy of Sciences, Beijing, 100101, China;
c. Inner Mongolia Mengwei Biotech Co. Ltd, Hohhot, 012000, China;
d. University of Chinese Academy of Sciences, Beijing, 100049, China;
e. College of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010080, China

Corresponding author: Yuhai Bi, beeyh@im.ac.cn
Wei Wang, wangwei@imu.edu.cn
Received Date: 15 October 2022
Accepted Date: 28 September 2023
Available online: 29 September 2023

Abstract

Highlights
1. A novel Akabane virus strain LK07 was isolated from cattle.
2. Here, we firstly reported the identification and isolation of the Akabane virus sub-lineage genogroup Ib in China.
3. The suckling mice showed clinical symptoms after intracerebrally infected with LK07 strain.
4. Viral antigen was detected in brain of mice infected with LK07 strain via IC, IP and SC routes.

Electronic Supplementary Material

10.1016j.virs.2023.09.006-ESM.docx
References
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11. Oem, J.K., Yoon, H.J., Kim, H.R., Roh, I.S., Lee, K.H., Lee, O.S., Bae, Y.C., 2012. Genetic and pathogenic characterization of Akabane viruses isolated from cattle with encephalomyelitis in Korea. Vet Microbiol. 158, 259-66.
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Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice

Corresponding author: Yuhai Bi, beeyh@im.ac.cn

Corresponding author: Wei Wang, wangwei@imu.edu.cn

a. State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, 010070, China;

b. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), CAS-TWAS Center of Excellence for Emerging Infectious Diseases (CEEID), Chinese Academy of Sciences, Beijing, 100101, China;

c. Inner Mongolia Mengwei Biotech Co. Ltd, Hohhot, 012000, China;

d. University of Chinese Academy of Sciences, Beijing, 100049, China;

e. College of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010080, China

Received Date: 15 October 2022

Accepted Date: 28 September 2023

Abstract: Highlights
1. A novel Akabane virus strain LK07 was isolated from cattle.
2. Here, we firstly reported the identification and isolation of the Akabane virus sub-lineage genogroup Ib in China.
3. The suckling mice showed clinical symptoms after intracerebrally infected with LK07 strain.
4. Viral antigen was detected in brain of mice infected with LK07 strain via IC, IP and SC routes.

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Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice

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