Citation: Xiaohui Zan, Shirong Wang, Tianqi Zhang, Ying Li, Chunge Zhang, Cun Fu, Jialei Wang, Youzhi Wu, Yanhua Ma, Zhifei Li, Yan Wang, Hao Wang, Yuhai Bi, Wei Wang. Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice .VIROLOGICA SINICA, 2023, 38(6) : 966-970.  http://dx.doi.org/10.1016/j.virs.2023.09.006

Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice

  • Corresponding author: Yuhai Bi, beeyh@im.ac.cn
    Wei Wang, wangwei@imu.edu.cn
  • Received Date: 15 October 2022
    Accepted Date: 28 September 2023
    Available online: 29 September 2023
  • Highlights
    1. A novel Akabane virus strain LK07 was isolated from cattle.
    2. Here, we firstly reported the identification and isolation of the Akabane virus sub-lineage genogroup Ib in China.
    3. The suckling mice showed clinical symptoms after intracerebrally infected with LK07 strain.
    4. Viral antigen was detected in brain of mice infected with LK07 strain via IC, IP and SC routes.

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  • 10.1016j.virs.2023.09.006-ESM.docx
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    5. Gao, H.F., Wang, J.P., Yang, Z.X., Xie, J.R., He, Y.W., Hong, Q.H., Xin, A.G., 2022. Genetic and Pathogenic Characterisation of a Virulent Akabane Virus Isolated from Goats in Yunnan, China. J Vet Res. 66, 35-42.

    6. Kimura, K., Yanase, T., Kato, T, 2021. Histopathological, Immunohistochemical and In-Situ Hybridization Findings in Suckling Rats Experimentally Infected With Akabane Genogroups Ⅰ and Ⅱ, Aino and Peaton Viruses. J Comp Pathol 187:27-39.

    7. Kono, R., Hirata, M., Kaji, M., Goto, Y., Ikeda, S., Yanase, T., Kato, T., Tanaka, S., Tsutsui, T., Imada, T., Yamakawa, M., 2008. Bovine epizootic encephalomyelitis caused by Akabane virus in southern Japan. BMC Vet Res. 4, 20.

    8. Kurogi, H., Inaba, Y., Takahashi, E., Sato, K., Omori, T., Miura, Y., Goto, Y., Fujiwara, Y., Hatano, Y., Kodama, K., Fukuyama, S., Sasaki, N., Matumoto, M., 1976. Epizootic congenital arthrogryposis-hydranencephaly syndrome in cattle: isolation of Akabane virus from affected fetuses. Arch Virol. 51, 67-74.

    9. Liao, Y.K., Lu, Y.S., Goto, Y., Inaba, Y. 1996. The isolation of Akabane virus (Iriki strain) from calves in Taiwan. J Basic Microbiol. 36, 33-9.

    10. Murata, Y., Uchida, K., Shioda, C., Uema, A., Bangphoomi, N., Chambers, J.K., Akashi, H., Nakayama, H., 2015. Histopathological Studies on the Neuropathogenicity of the Iriki and OBE-1 Strains of Akabane Virus in BALB/cAJcl Mice. J Comp Pathol.153 (2-3), 140-9.

    11. Oem, J.K., Yoon, H.J., Kim, H.R., Roh, I.S., Lee, K.H., Lee, O.S., Bae, Y.C., 2012. Genetic and pathogenic characterization of Akabane viruses isolated from cattle with encephalomyelitis in Korea. Vet Microbiol. 158, 259-66.

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    13. Purnomo, E.S., Ibrahim, A., Sukoco, R., Bunali, L., Taguchi, M., Kato, T., Yanase, T., Shirafuji, H., 2017. Molecular characterization of an Akabane virus isolate from West Java, Indonesia. J Vet Med Sci. 79, 774-779.

    14. Sevik, M., 2017. Molecular detection and genetic analysis of Akabane virus genogroup Ib in small ruminants in Turkey. Arch Virol. 162, 2769-2774.

    15. Takenaka-Uema, A., Matsugo, H., Ohira, K., Sekine, W., Murakami, S., Horimoto, T., 2022. Different organ and tissue tropism between Akabane virus genogroups in a mouse model. Virus Res. 314, 198752.

    16. Tang, H.B., Ren, P., Qin, S., Lin, J., Bai, A., Qin, S., Chen, F., Liu, J., Wu, J., 2019. Isolation, genetic analysis of the first Akabane virus from goat in China. J Vet Med Sci. 81,1445-1449.

    17. Uchida, K., Murakami, T., Sueyoshi, M., Tsuda, T., Inai, K., Acorda, J.A., Yamaguchi, R., Tateyama, S.,2000. Detection of Akabane viral antigens in spontaneous lymphohistiocytic encephalomyelitis in cattle. J Vet Diagn Invest. 12, 518-24.

    18. Wang, J., Blasdell, K.R., Yin, H., Walker, P.J., 2017. A large-scale serological survey of Akabane virus infection in cattle, yak, sheep and goats in China. Vet Microbiol. 207, 7-12.

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    Isolation and characterization of Akabane virus Ib substrain and its pathogenesis in sulking mice

      Corresponding author: Yuhai Bi, beeyh@im.ac.cn
      Corresponding author: Wei Wang, wangwei@imu.edu.cn
    • a. State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, College of Life Sciences, Inner Mongolia University, Hohhot, 010070, China;
    • b. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Center for Influenza Research and Early-warning (CASCIRE), CAS-TWAS Center of Excellence for Emerging Infectious Diseases (CEEID), Chinese Academy of Sciences, Beijing, 100101, China;
    • c. Inner Mongolia Mengwei Biotech Co. Ltd, Hohhot, 012000, China;
    • d. University of Chinese Academy of Sciences, Beijing, 100049, China;
    • e. College of Basic Medicine, Inner Mongolia Medical University, Hohhot, 010080, China

    Abstract: Highlights
    1. A novel Akabane virus strain LK07 was isolated from cattle.
    2. Here, we firstly reported the identification and isolation of the Akabane virus sub-lineage genogroup Ib in China.
    3. The suckling mice showed clinical symptoms after intracerebrally infected with LK07 strain.
    4. Viral antigen was detected in brain of mice infected with LK07 strain via IC, IP and SC routes.

    Reference (19)

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