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Volume 01 Issue 2
April 1986
    Citation: LUO Jing, WANG Han-Zhong, YANG Xue-Lou, WU Xi-Ying, HUANG Wen-Lin. A STUDY ON THE NPV OF ARGROGRAMMA AGNATA STGR AND ANALYSIS OF ITS NUCLEAR ACID MAP WITH RESTRICTION ENDONUCLEASES .VIROLOGICA SINICA, 1986, 1(2) : 24.
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    A STUDY ON THE NPV OF ARGROGRAMMA AGNATA STGR AND ANALYSIS OF ITS NUCLEAR ACID MAP WITH RESTRICTION ENDONUCLEASES

    • 1.

      Wuhan Insititute of Virology

    • 2.

      Academia Sinica

    • Available online: 05 June 1986
    • In this paper the morphology of NPV isolated from A.a.Stgr.is des- cribed and the map of A.a.NPV-DNA extracted in a simple method is obtained through restriction endonuclease digest such as EcoRI,BglⅠ, BglⅡ,BamHI,HindⅢ,and double digest BglⅠ+BglⅡ,BamHI+EcoRI.By using relationship between the different size of λ-DNA cleaved by EcoRI and it s mobility in 1% agarose electrophosis,standard curve of molec- ular weight is made.The molecular weight of each A.a.NPV-DNA fragments generated by restriction endonucleas...
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      A STUDY ON THE NPV OF ARGROGRAMMA AGNATA STGR AND ANALYSIS OF ITS NUCLEAR ACID MAP WITH RESTRICTION ENDONUCLEASES

      • 1. Wuhan Insititute of Virology
      • 2. Academia Sinica

      Abstract: In this paper the morphology of NPV isolated from A.a.Stgr.is des- cribed and the map of A.a.NPV-DNA extracted in a simple method is obtained through restriction endonuclease digest such as EcoRI,BglⅠ, BglⅡ,BamHI,HindⅢ,and double digest BglⅠ+BglⅡ,BamHI+EcoRI.By using relationship between the different size of λ-DNA cleaved by EcoRI and it s mobility in 1% agarose electrophosis,standard curve of molec- ular weight is made.The molecular weight of each A.a.NPV-DNA fragments generated by restriction endonucleas...

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