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Volume 01 Issue 4
August 1986
    Citation: GAO Qian, JIANG Chao-Zhun, MA Wen-Yu, ZHONG Fang-Yang, CA Xiao-Ning, HONG Mei-Xian. THE PRODUCTION OF AN ANTI-NSV-1 LYMPHOCYTE HYBRIDOMA CELL LINE AND TYPING OF HSV ISOLATES WITH MONOCLONAL AHTIBODIES .VIROLOGICA SINICA, 1986, 1(4) : 43.
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    THE PRODUCTION OF AN ANTI-NSV-1 LYMPHOCYTE HYBRIDOMA CELL LINE AND TYPING OF HSV ISOLATES WITH MONOCLONAL AHTIBODIES

    • 1.

      Dept. Microbiology

    • 2.

      The Fourth Military Medical College

    • 3.

      Xian

    • Available online: 05 December 1986
    • A hybridoma cell line(Mad-2) was produced by fusing mouse myloma cell line Sp2/O with the spleen cells immunized by HSV-1 SM44 strain infected BHK-21 cells and membrane protein antigen extracted from the HSV-1 infected ceils, the positive clone Mad-2 secreating monoclonal antibody(McAb) against HSV-1 was selected by ELISA. the supernatant and ascites producet by Mad-2 all reacted with HSV-1, hut not with HSV-2. the titer of McAb in ascites mesured by ELISA was 10~(-7) and the subclass of the Mad-2 was IgG1....
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      THE PRODUCTION OF AN ANTI-NSV-1 LYMPHOCYTE HYBRIDOMA CELL LINE AND TYPING OF HSV ISOLATES WITH MONOCLONAL AHTIBODIES

      • 1. Dept. Microbiology
      • 2. The Fourth Military Medical College
      • 3. Xian

      Abstract: A hybridoma cell line(Mad-2) was produced by fusing mouse myloma cell line Sp2/O with the spleen cells immunized by HSV-1 SM44 strain infected BHK-21 cells and membrane protein antigen extracted from the HSV-1 infected ceils, the positive clone Mad-2 secreating monoclonal antibody(McAb) against HSV-1 was selected by ELISA. the supernatant and ascites producet by Mad-2 all reacted with HSV-1, hut not with HSV-2. the titer of McAb in ascites mesured by ELISA was 10~(-7) and the subclass of the Mad-2 was IgG1....

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