DETECTION OF HEPATITIS A ANTIGEN BY A CELL CULTURE FLUORESCENT ANTIBODY TECHNIQUE WITH BIOTIN-AVIDIN

This paper describes an improved fluorescent antibody technique for the detection of HAV. The assay is unique in its use of the characteristic of a highaffinity complex between fluorescence-conjugated avidin and biotin-linked anti-HAV to increase sensitivity. We have made a comparison between direct-fluorescent and fluorescentantibody technique with biotin-avidin with same lot IgG antibody and same lot ACMK inoculated HAV. Because of the high binding affinity of this system due to multiple binding of biotin...