Citation: ZHANG Xiang-Ming, ZHANG Guo-Ming, ZHAO Yan-Ping, SHU Yong-Xin. Analysis of Japanese Encephalitis Viral Structural Polypeptides by SDS-PAGE Silver Staining .VIROLOGICA SINICA, 1988, 3(2) : 131.

Analysis of Japanese Encephalitis Viral Structural Polypeptides by SDS-PAGE Silver Staining

  • Available online: 05 March 1988
  • Japanese encephalitis virus SA14 strain(JEV) precipitated by 9% PEG 6000 from cultural medium of C_3/36 cells has been purified by centrifugation through a 20% and 60% sucrose discontinuous gradient. The polypeptides of JEV-SA14 strain were analysed in a discontinuous SDS-PAGE silver staining system. Three structural polypeptides of JEV-SA14 strain with MW of 53,000(VP_3), 12,200(VP_2) and 9,200(VP_1) were identified respectively. These structural polypeptides presenting in the form of monomer were demonstr...

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    Analysis of Japanese Encephalitis Viral Structural Polypeptides by SDS-PAGE Silver Staining

    • 1. National Institute for the Control of Pharmaceutical and Biological Products
    • 2. Beijing

    Abstract: Japanese encephalitis virus SA14 strain(JEV) precipitated by 9% PEG 6000 from cultural medium of C_3/36 cells has been purified by centrifugation through a 20% and 60% sucrose discontinuous gradient. The polypeptides of JEV-SA14 strain were analysed in a discontinuous SDS-PAGE silver staining system. Three structural polypeptides of JEV-SA14 strain with MW of 53,000(VP_3), 12,200(VP_2) and 9,200(VP_1) were identified respectively. These structural polypeptides presenting in the form of monomer were demonstr...

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