Citation: LIN Xiang-Dong, Ge-Chi-Hua, Tu-Chuan-Lin, HU Xin-Mei, ZHANG Hui-Xing, GAO Li, ZHOU Gong. Cytomegalovirus Neutral Red Plaque Assay And High Titer Virus Preparation .VIROLOGICA SINICA, 1989, 4(2) : 208.

Cytomegalovirus Neutral Red Plaque Assay And High Titer Virus Preparation

  • Available online: 05 June 1989
  • A neutral red plaque assay was established for the quantitation of infections cytomegalovirus(CMV), The plaque counts linearly correlated to the virus dilutions, indicating the accuracy of this method. Using of the conventional reagents and instruments and quicker results (8 days)may facilitate the application of the assay, With this assay, it was found that rotating culture of CMV-infected cells could easily produce infectious CMV titer as high as 1.8×10~7 PFU/ml, when the culture medium supernatant was co...

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    Cytomegalovirus Neutral Red Plaque Assay And High Titer Virus Preparation

    • 1. Department of Microbiology
    • 2. Shanghai Medical University
    • 3. Shanghai

    Abstract: A neutral red plaque assay was established for the quantitation of infections cytomegalovirus(CMV), The plaque counts linearly correlated to the virus dilutions, indicating the accuracy of this method. Using of the conventional reagents and instruments and quicker results (8 days)may facilitate the application of the assay, With this assay, it was found that rotating culture of CMV-infected cells could easily produce infectious CMV titer as high as 1.8×10~7 PFU/ml, when the culture medium supernatant was co...

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