Citation: CHAN Yi-Fei, JI Hong, WU Zheng-Quan, LV Mei-Zhen, ZHENG Bao-Fen, CHENG Li, KONG Xian-Shou. The Purifications of Mouse Ascitic Tumor Virus(SRSV) and Viral Ribonucleic Acid(SRSV RNA)and Investigation of Some Properties .VIROLOGICA SINICA, 1989, 4(4) : 350.

The Purifications of Mouse Ascitic Tumor Virus(SRSV) and Viral Ribonucleic Acid(SRSV RNA)and Investigation of Some Properties

  • Available online: 05 December 1989

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    The Purifications of Mouse Ascitic Tumor Virus(SRSV) and Viral Ribonucleic Acid(SRSV RNA)and Investigation of Some Properties

    • 1. Department of Biophysics and Pathophysiology
    • 2. Shanghai Medical University
    • 3. Shanghai

    Abstract: SRSV was obtained from the supernatant of infected NIH 3T3 mouse fiblasts and purified by sucrose density gradient centrif ugation.SRSV RNA was prepared from fresh viral pellets and extracted with chloroform-phen(?)l and fractionated on sucrose gradients. The RNAs of various fractions were determined by sedimeptation coefficient(s) and followed by gel electrophoresis. The component of SRSV RNA contains two main fractions, one sedimenting at about 60-70s(after treatment with heat at about 35S), plus small am...

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