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Volume 06 Issue 2
April 1991
    Citation: LIU Yan-Fang, YANG Shou-Jing, YAN Pei-Song, WANG Chun-Jie. Detection in Situ and Partial Diffusion of Epidemic Hemorrhagic Fever Viral Antigen in Cells .VIROLOGICA SINICA, 1991, 6(2) : 113.
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    Detection in Situ and Partial Diffusion of Epidemic Hemorrhagic Fever Viral Antigen in Cells

    • 1.

      Department of Pathology

    • 2.

      Fourth Military Medical University Xian

    • Available online: 05 June 1991
    • In the detecton of epidemic hemmorrhagic fever viral antigen in the autopsy and biopsy tissues by immunocytochemical methods, it was found that the monoclonal antibodies to nuclear antigen could stain antigen well with a rather diffuse pattern in fresh and well fixed material only, Polyclonal antibody could stain autopsy material imbedded in paraffin for even 28 years, as clear-cut vescular inclusion body. Probably the N antigen has been diffused away after long preservation and bad fixation. ABC, four fold...
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      Detection in Situ and Partial Diffusion of Epidemic Hemorrhagic Fever Viral Antigen in Cells

      • 1. Department of Pathology
      • 2. Fourth Military Medical University Xian

      Abstract: In the detecton of epidemic hemmorrhagic fever viral antigen in the autopsy and biopsy tissues by immunocytochemical methods, it was found that the monoclonal antibodies to nuclear antigen could stain antigen well with a rather diffuse pattern in fresh and well fixed material only, Polyclonal antibody could stain autopsy material imbedded in paraffin for even 28 years, as clear-cut vescular inclusion body. Probably the N antigen has been diffused away after long preservation and bad fixation. ABC, four fold...

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