The Establishment and Application of Immunofluorescence Assay for Detection of Antibodies to HIV-1
Abstract: Using the mixture of HIV -1 infected and no - infected HeLa CD4+ cells, the antigen slides were prepared and the immunofluorescence assay for detection of antibodies to human immunode-ficiency virus type 1 was established. Seventy - seven serum samples including the national stan-dard reference of HIV antibody reagents were tested. The sensitivity and specificity were 96. 4 %and 100%, respectively. Compared with the confirmation test of Western Blot, the coincident rate was 92. 6 %. The results indicated th...