Studies on some immunological properties of HHV-6 infected cells

The local strain of human herpesvirus 6 (HHV 6),named CN5, was used to study some immunological properties of infected cells.By the methods of indirect immunofluorescence test, APAAP and MTT, the morphological and kinetic character of viral antigen expression, the changes of CD antigens expressions and the cellular proliferation reaction induced by PHA were detected. The viral antigens were appeared in cord blood mononuclear cells (CBMCs) 8-12 hours after infection. By 48 hours positive cells reached 36%. After CN5 infection, in both cases of CBMCs and adult peripheral blood mononuclear cells (PBMCs), the CD3 positive cells decreased while the CD4 positive cells increased. No significant changes were found in the percentages of CD2, CD8 and CD45 RA positive cells. CN5 infected cell lysates inhibited the proliferation of PBMCs induced by PHA in a protein concentration dependent pattern, and this inhibition could be partially eradicated by the antisera to HHV 6.