Citation: HAN Xin-Bing, ZHANG Fu-Ping, WANG Zheng-Dang, HUANG Jia-Si. Detection of group B rotavirus by RT-PCR .VIROLOGICA SINICA, 1998, 13(3) : 279.

Detection of group B rotavirus by RT-PCR

  • Available online: 10 June 2015
  • A diagnostic assay was presented to detect group B rotavirus (GBRV) in fecal specimens with reverse transcription polymerase chain reaction (RT PCR). GBRV double strand RNAs (dsRNA) isolated from stool samples were reverse transcribed and amplified by PCR using two oligonucleotide primers which were derived from genomic segment 3 of the IDIR (intestinal disease of infants rat) strain of GBRV. This RT PCR assay permitted the sensitive and specific detection of a variety of GBRV in fecal specimens. Wild GBRV strains of lamb, kid were detected with these primer pairs by RT PCR. Moreover, RT PCR also permitted the detection of genomic RNA of lamb GBRV KB 63 strain which has been adapted to serial passage in cattle. The specific PCR products of 290 bp suggested that GBRV from lamb, kid and calf had identical sequences in genomic RNA;

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    Detection of group B rotavirus by RT-PCR

    • 1. Faculty of Animal Medicine.Xinjiang Agricultural University.Urumqi,Xinjiang 830052 State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100080

    Abstract: A diagnostic assay was presented to detect group B rotavirus (GBRV) in fecal specimens with reverse transcription polymerase chain reaction (RT PCR). GBRV double strand RNAs (dsRNA) isolated from stool samples were reverse transcribed and amplified by PCR using two oligonucleotide primers which were derived from genomic segment 3 of the IDIR (intestinal disease of infants rat) strain of GBRV. This RT PCR assay permitted the sensitive and specific detection of a variety of GBRV in fecal specimens. Wild GBRV strains of lamb, kid were detected with these primer pairs by RT PCR. Moreover, RT PCR also permitted the detection of genomic RNA of lamb GBRV KB 63 strain which has been adapted to serial passage in cattle. The specific PCR products of 290 bp suggested that GBRV from lamb, kid and calf had identical sequences in genomic RNA;

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