Citation: LI Xin, QIN Qilian, DING Cui, LIU Haihong. 李馨 ,秦启联卜 ,丁翠 ,刘海虹 .VIROLOGICA SINICA, 2001, 16(4) : 373-376.

李馨 ,秦启联卜 ,丁翠 ,刘海虹

  • Available online: 05 December 2001
  • A polydnavirus(PDV)was purified by sucrose density gradient centrifugation from the calyx region of female parasitoid wasp Microplitis mediator (Hymenoptera:Braconidae).Negative stain technique showed that the virus was tadpole like and measured 130×35nm.A diverse protein profile of the virus’capsid proteins was displayed by SDS PAGE.Its genome was composed of over 14 DNA molecules,which were different in molecular size and abundance.Molecular size of the genome was approximate 108 kbp calculated by patterns from six restrict endonucleases digestion.Micro injection experiment with calyx fluid of the female wasps showed that the virus suppressed growth and development of host of the wash.

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    李馨 ,秦启联卜 ,丁翠 ,刘海虹

    • 1. 北京 农业虫害鼠害综合治理研究国家重点实验室 中国科学院动物研究所 中国农业大学电镜室

    Abstract: A polydnavirus(PDV)was purified by sucrose density gradient centrifugation from the calyx region of female parasitoid wasp Microplitis mediator (Hymenoptera:Braconidae).Negative stain technique showed that the virus was tadpole like and measured 130×35nm.A diverse protein profile of the virus’capsid proteins was displayed by SDS PAGE.Its genome was composed of over 14 DNA molecules,which were different in molecular size and abundance.Molecular size of the genome was approximate 108 kbp calculated by patterns from six restrict endonucleases digestion.Micro injection experiment with calyx fluid of the female wasps showed that the virus suppressed growth and development of host of the wash.

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