李馨 ，秦启联卜 ，丁翠 ，刘海虹

A polydnavirus(PDV)was purified by sucrose density gradient centrifugation from the calyx region of female parasitoid wasp Microplitis mediator (Hymenoptera:Braconidae).Negative stain technique showed that the virus was tadpole like and measured 130×35nm.A diverse protein profile of the virus’capsid proteins was displayed by SDS PAGE.Its genome was composed of over 14 DNA molecules,which were different in molecular size and abundance.Molecular size of the genome was approximate 108 kbp calculated by patterns from six restrict endonucleases digestion.Micro injection experiment with calyx fluid of the female wasps showed that the virus suppressed growth and development of host of the wash.