Citation: GAO Juan, YANG Shoujing, SUN Yu-jing, LIU Yan—fang. The Interaction of Viral Nucleocapsid Protein with GRP94 and HSP27 in Brains of Suckling M ice Experimentally Infected with Hantaan virus .VIROLOGICA SINICA, 2003, 18(2) : 104-107.

The Interaction of Viral Nucleocapsid Protein with GRP94 and HSP27 in Brains of Suckling M ice Experimentally Infected with Hantaan virus

  • Available online: 15 April 2003
  • To study the association of heat-shock protein GRP94 an d HSP27 with Hantaan virus nucleocapsid protein(HTNV—NP)during viral replication,new—bom mice less than 72h of age were experimentally infected with Hantaan virus intracerebrally.Brains of mi ce at the 8th day post-infection were prepared for tissue sections an d extracts.Hantaan virus infection,GRP94,HSP27 expression an d their localization were demonstrated by immunohistochemi cal staining.The possible interaction between HTNV—NP an d GRIX)4.HSP27 was an alyzed by ELISA,immunoprecipitation.HTNV infection led to increased expression of GRIX)4 but not HSP27 at cytoplasma of neurons in brains of infected suckling mice.colocalizationof GRIX)4 with HTNV—NP,an d association of both GRP94 and HSP27 with HTNV—NP.Th e results provide the basis for further research of the roles of HSPs in the viral infection and replication.

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    The Interaction of Viral Nucleocapsid Protein with GRP94 and HSP27 in Brains of Suckling M ice Experimentally Infected with Hantaan virus

    • 1. Department of Pathology,the Fourth Military Medical University,Xi'an 710032,China

    Abstract: To study the association of heat-shock protein GRP94 an d HSP27 with Hantaan virus nucleocapsid protein(HTNV—NP)during viral replication,new—bom mice less than 72h of age were experimentally infected with Hantaan virus intracerebrally.Brains of mi ce at the 8th day post-infection were prepared for tissue sections an d extracts.Hantaan virus infection,GRP94,HSP27 expression an d their localization were demonstrated by immunohistochemi cal staining.The possible interaction between HTNV—NP an d GRIX)4.HSP27 was an alyzed by ELISA,immunoprecipitation.HTNV infection led to increased expression of GRIX)4 but not HSP27 at cytoplasma of neurons in brains of infected suckling mice.colocalizationof GRIX)4 with HTNV—NP,an d association of both GRP94 and HSP27 with HTNV—NP.Th e results provide the basis for further research of the roles of HSPs in the viral infection and replication.

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