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Volume 19 Issue 4
August 2004
    Citation: XU Li-hua, SU Xin-ming, LIU Hua-lei, REN Xue-feng, WANG Zhi-liang, WU Yan-gong, CHEN Pu-yan. Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and Respiratory Syndrome Virus .VIROLOGICA SINICA, 2004, 19(4) : 401-403.
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    Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and Respiratory Syndrome Virus

    • 1.

      1. Key Laboratory of Animal Disease Diagnosis and Immunology Nanjing Agricultural University，Nanjing 210095，China

    • 2.

      Department of Animal Science,Agricultural College of Ningxia University，Yinchuan 750021，China

    • 3.

      Animal Quarantine Institute of Agriculture Ministry，QingDao 266032，China

    • Corresponding author: CHEN Pu-yan, 
    • Available online: 25 August 2004
    • The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration，temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the method’s sensitivity, specificity and stability were confirmed. About 200 serum samples were detected by using the method, and these samples were also detected by recombinant N protein based ELISA and IDEXX ELISA kit. The result showed that the agreement ratio of the recombinant N protein based LAT with other two methods arrived at 93%, 78%, respectively.
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      Establishment of Recombinant N Protein Based Latex Agglutination Test for Detection of Antibody Against Porcine Reproductive and Respiratory Syndrome Virus

        Corresponding author: CHEN Pu-yan,
      • 1. 1. Key Laboratory of Animal Disease Diagnosis and Immunology Nanjing Agricultural University，Nanjing 210095，China
      • 2. Department of Animal Science,Agricultural College of Ningxia University，Yinchuan 750021，China
      • 3. Animal Quarantine Institute of Agriculture Ministry，QingDao 266032，China

      Abstract: The purified recombinant nuclocapsid protein of PRRSV was used to conjoined with latex, and the conjoined concentration，temperature and time were optimized, a detection method for serum antibodies against Porcine reproductive and respiratory syndrome virus was established. Some tests for the method’s sensitivity, specificity and stability were confirmed. About 200 serum samples were detected by using the method, and these samples were also detected by recombinant N protein based ELISA and IDEXX ELISA kit. The result showed that the agreement ratio of the recombinant N protein based LAT with other two methods arrived at 93%, 78%, respectively.

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