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Volume 23 Issue 3
June 2008

    Li-na ZHU, Chun-lei MEI and Zhong-xin ZHANG. The Function of SeMNPV IAP3 in Mammalian Cells[J]. Virologica Sinica, 2008, 23(3): 183-188. doi: 10.1007/s12250-008-2883-5
    Citation: Li-na ZHU, Chun-lei MEI, Zhong-xin ZHANG. The Function of SeMNPV IAP3 in Mammalian Cells .VIROLOGICA SINICA, 2008, 23(3) : 183-188.  http://dx.doi.org/10.1007/s12250-008-2883-5
    shu

    甜菜夜蛾核型多角体病毒IAP3在哺乳动物细胞中功能的研究

    cstr: 32224.14.s12250-008-2883-5
    • 1.

      中国科学院武汉病毒研究所，2 中国科学院研究生院

    • 通讯作者： 张忠信, zhangzx@wh.iov.cn
    • 收稿日期： 2007-05-25
      录用日期： 2008-01-04

    • 杆状病毒抑制细胞凋亡蛋白（inhibitor of apoptosis protein，IAP）在病毒的感染性和宿主范围上起重要作用。本文利用PCR方法，从甜菜夜蛾核型多角体病毒（SeMNPV）基因组中扩增出病毒的抑制细胞凋亡基因3（iap3），该基因克隆到pEGFP-C1质粒上，转化哺乳动物细胞HEK293，结果显示，甜菜夜蛾核多角体病毒IAP3的表达能明显抑制HEK293细胞由治疗肿瘤药物顺铂诱导的细胞凋亡。这首次证明了甜菜夜蛾核多角体病毒iap3在哺乳动物细胞中有抑制细胞凋亡的功能。

      Abstract

      The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene of Spodoptera exigua nucleopolyhedrovirus (SeMNPV), amplified by PCR, was 939 bp encoding IAP3. The PCR product was cloned into EcoR I /Bam H I of the plasmid pEGFP-C1. GFP was fused to the N-terminaus of IAP3 to study distribution in HEK293. It was observed that the plasmid expressing IAP3 significantly inhibited apoptosis induced by cisplatin in HEK293 cells. We conclude that the IAP3 of SeMNPV is functional in mammalian cells.

    The Function of SeMNPV IAP3 in Mammalian Cells

    • 1.

      State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    • 2.

      Graduate school, Chinese Academy of Sciences, Beijing 100039, China

    • Corresponding author: Zhong-xin ZHANG, zhangzx@wh.iov.cn
    • Received Date: 25 May 2007
      Accepted Date: 04 January 2008
    • The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene of Spodoptera exigua nucleopolyhedrovirus (SeMNPV), amplified by PCR, was 939 bp encoding IAP3. The PCR product was cloned into EcoR I /Bam H I of the plasmid pEGFP-C1. GFP was fused to the N-terminaus of IAP3 to study distribution in HEK293. It was observed that the plasmid expressing IAP3 significantly inhibited apoptosis induced by cisplatin in HEK293 cells. We conclude that the IAP3 of SeMNPV is functional in mammalian cells.
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      1. Brinbaum M J, Clem R J, Miller L K. 1994. An apoptosis inhibiting gene from a nuclear polyhedrosis virus encoding a polypeptide with Cys/His sequence motifs. J Virol, 68: 2521-2528.

      2. Clem R J. 2001. Baculoviruses and apoptosis: the good, the bad and the ugly. Cell Death Differ, 8: 137-143.
          doi: 10.1038/sj.cdd.4400821

      3. Clem R J, Fechhemier M, Miller L K. 1991. Science 254: 1388-1390.

      4. Deveraux Q L, Reed J C. 1999. IAP family protein-supressors of apoptosis. Gene Dev, 13: 239-252
          doi: 10.1101/gad.13.3.239

      5. Du C, Fang M, Li Y, Li L, Wang X. 2000. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell, 102: 33-42.
          doi: 10.1016/S0092-8674(00)00008-8

      6. Duckett C S, Nava V E, Gedrich R W, et al. 1996. A conserved family of cellular genes related to the baculo-virus iap gene and encoding apoptosis inhibitors. EMBO J, 15: 2685-2694.

      7. Hawkins C J, Uren A G, Hacker G, et al. 1996. Inhibition of interleukin 1β-converting enzyme-mediated apoptosis of mammalian cells by baculovirus IAP. Proc Natl Acad Sci USA, 93 : 13786-13790.
          doi: 10.1073/pnas.93.24.13786

      8. Hay B A, Wassarman D A, Rubin G M. 1995. Drosophila homologs of baculovirus inhibitor of apoptosis protein function to block cell death. Cell, 83 : 1253-1262.
          doi: 10.1016/0092-8674(95)90150-7

      9. Hozak R R, Manji G A, Friesen P D. 2000. The BIR motifs mediate dominant interference and oligomerization of inhibitor of apoptosis Op-IAP. Mol Cell Biol, 20: 1877-1885.
          doi: 10.1128/MCB.20.5.1877-1885.2000

      10. Liu X, Kirn C N, Yang J, et al. 1996. Induction of apoptotic program in cell-free abstracts: requirement for dIAP and cytochrome c. Cell 86: 147-157.
          doi: 10.1016/S0092-8674(00)80085-9

      11. Miller LK. 1999. An exegesis of IAPs: salvation and surprises from BIR motifs. Trends Cell Biol, 9: 323-328.
          doi: 10.1016/S0962-8924(99)01609-8

      12. O'Brien V. 1998. Viruses and apoptosis. J Gen Virol, 79 : 1833-1845.
          doi: 10.1099/0022-1317-79-8-1833

      13. Roulson A, Marcellus R C, Banton P E. 1999. Viruses and apoptosis. Annu Rev Microbiol, 53: 577-628.
          doi: 10.1146/annurev.micro.53.1.577

      14. Seshagiri S, Miller L K. 1997. Baculovirus inhibitors of apoptosis (IAPs) block activation of Sf-caspase-1. Cell Biology, 94: 13606-13611.

      15. Uren A G, Pakusch M, Hawkina C J, et al. 1996. Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors. Proc Natl Acad Sci USA, 93: 4974-4978.
          doi: 10.1073/pnas.93.10.4974

      16. Verhagen A M, Ekert P G, Pakusch M, et al. 2000. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell, 102: 43-53.
          doi: 10.1016/S0092-8674(00)00009-X

      17. Vucic D, Kaiser W J, Harvey A J, et al. 1997. Inhibition of reaper-induced apoptosis by interaction with inhibitor of apoptosis proteins (IAPs). Proc Natl Acad Sci USA, 94 : 10183-10188.
          doi: 10.1073/pnas.94.19.10183

      18. Vucic D, Kaiser W J, Miller L K. 1998. Inhibitor of apoptosis proteins physically interact with and block apoptosis induced by Dosophila proteins HID and GRIM. Mol Cell Biol, 18: 3300-3309.
          doi: 10.1128/MCB.18.6.3300

      19. Lasa R, Ruiz-Portero C, Alcazar M D, et al. 2007, Efficacy of optical brighter formulation of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) as a biological insecticide in greenhouse in southern spain. Biol Control, 40: 80-96.
          doi: 10.1016/j.biocontrol.2006.09.004

      20. IJkel W F J, van Strien E A, Heldens J G M, et al. 1999. Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. J Gen Virol, 80: 3289-3304.
          doi: 10.1099/0022-1317-80-12-3289

      21. Sauerwald T M, Oyler G A, Betenbaugh M J. 2003, Study of caspase inhibitors for limiting death in mam-malian cell culture. Biotechnol Bioeng, 81: 329-340.
          doi: 10.1002/(ISSN)1097-0290

      22. Niu G D and Zhang Z X. 2004, Interaction between Ubiqutin and IAP2 or IAP3 of Spodoptera exigua nucleopolyhedrovirus. Virologica Sinca, 19: 49-53. (in Chinese)

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      The Function of SeMNPV IAP3 in Mammalian Cells

        Corresponding author: Zhong-xin ZHANG, zhangzx@wh.iov.cn
      • 1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
      • 2. Graduate school, Chinese Academy of Sciences, Beijing 100039, China
      • Received Date: 25 May 2007
      • Accepted Date: 04 January 2008

      Abstract: The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene of Spodoptera exigua nucleopolyhedrovirus (SeMNPV), amplified by PCR, was 939 bp encoding IAP3. The PCR product was cloned into EcoR I /Bam H I of the plasmid pEGFP-C1. GFP was fused to the N-terminaus of IAP3 to study distribution in HEK293. It was observed that the plasmid expressing IAP3 significantly inhibited apoptosis induced by cisplatin in HEK293 cells. We conclude that the IAP3 of SeMNPV is functional in mammalian cells.

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