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Volume 23 Issue 1
February 2008

    Wei LI, Yuan-ying SHEN, Xuan-rong ZHANG, Lai-feng REN, Qiang LI, Ru SHEN and Hai-ping ZHAO. Research on Hepatitis B virus Genotypes and Subgenotypes among Bai Nationality in Dali, Yunnan Province[J]. Virologica Sinica, 2008, 23(1): 57-62. doi: 10.1007/s12250-008-2923-1
    Citation: Wei LI, Yuan-ying SHEN, Xuan-rong ZHANG, Lai-feng REN, Qiang LI, Ru SHEN, Hai-ping ZHAO. Research on Hepatitis B virus Genotypes and Subgenotypes among Bai Nationality in Dali, Yunnan Province .VIROLOGICA SINICA, 2008, 23(1) : 57-62.  http://dx.doi.org/10.1007/s12250-008-2923-1
    shu

    云南大理乙型肝炎病毒感染的基因型和亚型研究

    cstr: 32224.14.s12250-008-2923-1
    • 1.

      云南大理学院微生物学与免疫学教研室，云南大理 671000;2山西医科大学汾阳学院微生物学与免疫学教研室, 山西汾阳 032200

    • 通讯作者： 申元英, yuanyingshen@163.com; 
    • 收稿日期： 2007-11-22
      录用日期： 2007-12-26
    • 本研究采用了HBV基因型特异性引物分型法和限制性片段长度多态性（PCR-RFLP）方法分别对云南大理白族人群100例慢性HBV感染者进行HBV基因型和亚型研究。结果显示，100份血清标本中，B型41例(41%),全部为Ba亚型,未发现Bj亚型；C型25例(25%),其中Cs亚型21例(84%)，Ce亚型3例(12%)，未能分型1例(4%)；B+C 混合型34例(34%)。基因型B,C和B+C的分布在年龄和性别的构成无显著性差异（P=0.182；P=0.812）；在HBeAg阳性或HBeAb阳性的感染者中，HBV基因型的分布均无显著性差异（P=0.077；P=0.663）。研究表明，云南大理白族人群慢性HBV感染者中存在HBV的B型、B+C混合型和C型，其中B型是该人群的HBV优势基因型；基因亚型以Ba和Cs亚型为主要流行株。最显著的特点是B+C混合型感染占很大比例。

    Research on Hepatitis B virus Genotypes and Subgenotypes among Bai Nationality in Dali, Yunnan Province

    • 1.

      Department of Microbiology & Immunology, Dali University, Dali 671000, China

    • 2.

      Department of Microbiology & Immunology, Fenyang College of Shanxi Medical University, Fenyang 032200, China

    • Corresponding author: Yuan-ying SHEN, yuanyingshen@163.com
    • Received Date: 22 November 2007
      Accepted Date: 26 December 2007

      Fund Project: National Natural Science Foundation 30560136

    • To investigate the distribution of hepatitis B virus (HBV) genotypes and subgenotypes among the Bai nationality in Dali, a total of 100 serum samples from patients with chronic HBV-infection were collected for the detection of HBV genotypes and subgenotypes by genotype-specific primers and restriction fragment length polymorphism (RLFP), respectively. Among the 100 samples, the proportions of genotype B, C and mixed genotype (B+C) were 41%, 25% and 34%, respectively. All the genotype B strains belonged to subgenotype Ba. In genotype C, 84% were Subgenotype Cs and 12% were subgenotype Ce. The distribution of genotypes B, C and B+C showed no significant difference between male and female patients (P=0.182) and among the age groups of patients (P=0.812). The rates of HBeAg/HBeAg positivity were no significantly different among genotypes B, genotype C and mixed genotype (B+C) (P=0.077/P=0.663). In Dali, genotypes B, B+C and C existed among Bai nationality with chronic HBV-infection, and genotype B was the major genotype. Subgenotypes Ba and Cs were the predominant strains in patients with HBV genotype B/C infection. The most prominent characteristic was the higher prevalent rate of mixed genotype (B+C) in patients.
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      1. Chan H L, Tsui S K, Tse C H, et al. 2005. Epidemiological and virological characteristics of 2 subgroups of hepatitis B virus genotype C. J Infect Dis, 191: 2022-2032.
          doi: 10.1086/jid.2005.191.issue-12

      2. Funk M L, Rosenberg D M, Lok A S. 2002. World-wide epidemiology of HBeAg-negative chronic ehepatitis B and associated precore and core promoter variants. J Virol, 9: 52-61.

      3. Kao J H, Chen P J, Lai M Y, et al. 2002. Genotypes and clinical phenotypes of hepatitis B virus in patients with chronic hepatitis B virus infection. J Clin Microbiol, 40: 1207-1209.
          doi: 10.1128/JCM.40.4.1207-1209.2002

      4. Lim C K, Tan J T, Khoo J B, et al. 2006. Correlations of HBV Genotypes, Mutations Affecting HBeAg Expression and HBeAg/ anti-HBe Status in HBV Carriers. Int J Med Sci, 3: 14-20.

      5. Naito H, Hayashi S, Abe K. 2001. Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers. J Clin Microbiol, 39: 362-364.
          doi: 10.1128/JCM.39.1.362-364.2001

      6. Orito E, Mizokami M, Sakugawa H, et al. 2001. A case-control study for clinical and molecular biological difference between hepatitis B viruses of genotype B and C. Japan HBV Genotype research Group. Hepatology, 33: 218-223.
          doi: 10.1053/jhep.2001.20532

      7. Sugauchi F, Kumada H, Sakugawa H, et al. 2004. Two subtypes of genotype B(Ba and Bj) of hepatitis B virus in Japan. Clin Infect Dis, 38: 1222-1228.
          doi: 10.1086/382885

      8. Tanaka Y, Orito E, Yuen M F, et al.2005. Two subtypes (subgenotypes) of hepatiis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphism. Hepatol Res, 33: 216-224.

      9. Xia G L, Nainan O V, Jia Z Y, et al. 2001. Prevalence of mutants in the αdeterminant region of hepatitis B surface antigen among Chinese carriers after receiving only active postexposure immunoprophylaxis. Chinese J Exp Clin Vir, 15 (3): 212-215. (in Chinese)

      10. Zeng G, Wang Z, Wen S, et al. 2005. Geographic distribution, virologic and clinical characteristics of hepatitis B virus genotypes in China. J Viral Hepat, 12: 609-617.
          doi: 10.1111/jvh.2005.12.issue-6

      11. Zhao H, Li J, Li X F, et al. 2007. Clinical characteristics and distribution of hepatitis B virus genotype and subgenotype. Chin J Epidemiol, 28 (1): 74-77. (in Chinese)

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      Research on Hepatitis B virus Genotypes and Subgenotypes among Bai Nationality in Dali, Yunnan Province

        Corresponding author: Yuan-ying SHEN, yuanyingshen@163.com
      • 1. Department of Microbiology & Immunology, Dali University, Dali 671000, China
      • 2. Department of Microbiology & Immunology, Fenyang College of Shanxi Medical University, Fenyang 032200, China
      • Received Date: 22 November 2007
      • Accepted Date: 26 December 2007
      Fund Project:  National Natural Science Foundation 30560136

      Abstract: To investigate the distribution of hepatitis B virus (HBV) genotypes and subgenotypes among the Bai nationality in Dali, a total of 100 serum samples from patients with chronic HBV-infection were collected for the detection of HBV genotypes and subgenotypes by genotype-specific primers and restriction fragment length polymorphism (RLFP), respectively. Among the 100 samples, the proportions of genotype B, C and mixed genotype (B+C) were 41%, 25% and 34%, respectively. All the genotype B strains belonged to subgenotype Ba. In genotype C, 84% were Subgenotype Cs and 12% were subgenotype Ce. The distribution of genotypes B, C and B+C showed no significant difference between male and female patients (P=0.182) and among the age groups of patients (P=0.812). The rates of HBeAg/HBeAg positivity were no significantly different among genotypes B, genotype C and mixed genotype (B+C) (P=0.077/P=0.663). In Dali, genotypes B, B+C and C existed among Bai nationality with chronic HBV-infection, and genotype B was the major genotype. Subgenotypes Ba and Cs were the predominant strains in patients with HBV genotype B/C infection. The most prominent characteristic was the higher prevalent rate of mixed genotype (B+C) in patients.

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