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Volume 24 Issue 5
October 2009

    M. Berling, J. -B. Rey, S. -J. Ondet, Y. Tallot, O. Soubabère, A. Bonhomme, B. Sauphanor and M. Lopez-Ferber. Field Trials of CpGV Virus Isolates Overcoming Resistance to CpGV-M[J]. Virologica Sinica, 2009, 24(5): 470-477. doi: 10.1007/s12250-009-3056-x
    Citation: M. Berling, J. -B. Rey, S. -J. Ondet, Y. Tallot, O. Soubabère, A. Bonhomme, B. Sauphanor, M. Lopez-Ferber. Field Trials of CpGV Virus Isolates Overcoming Resistance to CpGV-M .VIROLOGICA SINICA, 2009, 24(5) : 470-477.  http://dx.doi.org/10.1007/s12250-009-3056-x
    shu

    Field Trials of CpGV Virus Isolates Overcoming Resistance to CpGV-M

    cstr: 32224.14.s12250-009-3056-x
    • 1.

      LGEI, Ecole des Mines d’Alès30319 AlèsFrance; 2GRABAgroparc, 84914 Avignon Cedex 9France; 3Arysta LifeScience, Route d’Artix, B.P80, 64150 NogueresFrance; 4NPP, Arysta LifeScience35, Avenue Léon Blum64000 Pau, France; 5Unité PSHINRAAgroparc, 84914 Avignon Cedex 9France

    • 收稿日期： 2009-01-31
      录用日期： 2009-05-31

    • The Cydia pomonella granulovirus (CpGV) has been used for many years as biological agent for codling moth control in apple orchards. Resistance to the Mexican strain of CpGV was detected in orchards in Germany, France and Italy. A laboratory insect colony was started from insects collected in a French resistant orchard. It was named RGV. Various virus isolates were identified as active against this resistant insect colony. Field tests were carried out in 2007 to test if the two virus isolates CpGV-I12 and NPP-R1 were effective in the field. Although these virus isolates were not able to reduce insect caused fruit damages, they significantly reduced the overwintering insect populations. NPP-R1 was subjected to eight passages on RGV larvae (NPP-R1.8) that improved its biological activity on RGV larvae. 2008 field trials were set up to test this improved virus strain, compared to CpGV-I12 and Madex plus active on RGV. These tests confirmed the ability to control both in susceptible and resistant insect populations.

    Field Trials of CpGV Virus Isolates Overcoming Resistance to CpGV-M

    • 1.

      LGEI, Ecole des Mines d'Alès. 30319 Alès. France

    • 2.

      GRAB. Agroparc, 84914 Avignon Cedex 9. France

    • 3.

      Arysta LifeScience, Route d'Artix, B.P. 80, 64150 Nogueres. France

    • 4.

      NPP, Arysta LifeScience. 35, Avenue Léon Blum. 64000 Pau, France

    • 5.

      Unité PSH. INRA. Agroparc, 84914 Avignon Cedex 9. France

    • Received Date: 31 January 2009
      Accepted Date: 31 May 2009
    • The Cydia pomonella granulovirus (CpGV) has been used for many years as biological agent for codling moth control in apple orchards. Resistance to the Mexican strain of CpGV was detected in orchards in Germany, France and Italy. A laboratory insect colony was started from insects collected in a French resistant orchard. It was named RGV. Various virus isolates were identified as active against this resistant insect colony. Field tests were carried out in 2007 to test if the two virus isolates CpGV-I12 and NPP-R1 were effective in the field. Although these virus isolates were not able to reduce insect caused fruit damages, they significantly reduced the overwintering insect populations. NPP-R1 was subjected to eight passages on RGV larvae (NPP-R1.8) that improved its biological activity on RGV larvae. 2008 field trials were set up to test this improved virus strain, compared to CpGV-I12 and Madex plus active on RGV. These tests confirmed the ability to control both in susceptible and resistant insect populations.
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      1. Asser-Kaiser S, Fritsch E, Undorf-Spahn K, et al. 2007. Rapid emergence of baculovirus resistance in codling moth due to dominant, sex-linked inheritance. Science, 317: 1916-1918.
          doi: 10.1126/science.1146542

      2. Berling M, Blachère-Lopez C, Soubabere O, et al. 2009. Cydia pomonella granulovirus (CpGV) genotypes overcome virus resistance in the codling moth and improve virus efficiency by selection against resistant hosts. Appl Env Microbiol, 75: 925-930.
          doi: 10.1128/AEM.01998-08

      3. Eberle K E, Asser-Kaiser S, Sayed S M, et al. 2008. Overcoming the resistance of codling moth against conventional Cydia pomonella granulovirus (CpGV-M) by a new isolate CpGV-I12. J Invertebr Pathol, 98: 293-298.
          doi: 10.1016/j.jip.2008.03.003

      4. Eberle K E, Jehle JA. 2006. Field resistance of codling moth against Cydia pomonella granulovirus (CpGV) is autosomal and incompletely dominant inherited. J Invertebr Pathol, 93: 201-206.
          doi: 10.1016/j.jip.2006.07.001

      5. Fritsch E, Undorf-Spahn K, Kienzle J, et al. 2005. Apfelwickler-Granulovirus: Erste Hinweise auf Unterschiede in der Empfindlichkeit lokaler Apfelwickler-Populationen (Codling moth granulovirus: First indication of variations in the susceptibility of local codling moth populations). Nachrichtenblatt des Deutschen Pflanzenschutzdienstes, 57: 29-34.

      6. Huber J. 1986. Use of baculoviruses in pest management programs. In: The biology of Baculoviruses (Granados R R, Federici B A. ed. ), Vol Ⅱ, Fl, Boca Raton: CRC Press. p181-202.

      7. Kienzle J, Zimmer J, Volk F, et al. 2008. Field tests with Madex Plus against CpGV-resistant codling moth populations in organic orchards in 2006. In: Ecofruit-13th International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-Growing: Proceedings to the Conference (Boos, Markus, Eds), Weinsberg, Germany. p339-349.

      8. Lacey L A, Thompson D, Vincent C, et al. 2008. Codding moth granulovirus: a comprehensive review. Biocontrol Sci Techn, 18: 639-663.
          doi: 10.1080/09583150802267046

      9. Rezapanah M, Shojai-Estabragh S, Hüber J, et al. 2008. Molecular and biological characterization of new isolates of Cydia pomonella granulovirus from Iran. J Pest Sci, 81: 187-191.
          doi: 10.1007/s10340-008-0204-2

      10. Sauphanor B, Berling M, Toubon J F, et al. 2006. Carpocapse des pommes: cas de résistance au virus de la granulose en vergers biologiques. Phytoma, la défense des végétaux, 590: 24-27.

      11. Tanada Y. 1964. A granuloses virus of the Codling moth, Carpocapsa pomonella (Linnaeus) (Olethreutidae, Lepi-doptera). J Invertebr Pathol, 6: 378-380.

      12. Zingg D. 2008. Madex Plus and Madex I12 overcome Virus Resistance of Codling Moth. In: Ecofruit-13th International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-Growing: Proceedings to the Conference (Boos, Markus, Eds), Weinsberg, Germany. p256-260.

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      Field Trials of CpGV Virus Isolates Overcoming Resistance to CpGV-M

      • 1. LGEI, Ecole des Mines d'Alès. 30319 Alès. France
      • 2. GRAB. Agroparc, 84914 Avignon Cedex 9. France
      • 3. Arysta LifeScience, Route d'Artix, B.P. 80, 64150 Nogueres. France
      • 4. NPP, Arysta LifeScience. 35, Avenue Léon Blum. 64000 Pau, France
      • 5. Unité PSH. INRA. Agroparc, 84914 Avignon Cedex 9. France
      • Received Date: 31 January 2009
      • Accepted Date: 31 May 2009

      Abstract: The Cydia pomonella granulovirus (CpGV) has been used for many years as biological agent for codling moth control in apple orchards. Resistance to the Mexican strain of CpGV was detected in orchards in Germany, France and Italy. A laboratory insect colony was started from insects collected in a French resistant orchard. It was named RGV. Various virus isolates were identified as active against this resistant insect colony. Field tests were carried out in 2007 to test if the two virus isolates CpGV-I12 and NPP-R1 were effective in the field. Although these virus isolates were not able to reduce insect caused fruit damages, they significantly reduced the overwintering insect populations. NPP-R1 was subjected to eight passages on RGV larvae (NPP-R1.8) that improved its biological activity on RGV larvae. 2008 field trials were set up to test this improved virus strain, compared to CpGV-I12 and Madex plus active on RGV. These tests confirmed the ability to control both in susceptible and resistant insect populations.

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