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Volume 24 Issue 6
December 2009

    Wen-hua ZHAO, Shi-biao YANG, Jian-qiang HAN, Mei JIANG, Hua-chun LI, Nian-zu ZHANG and Qi-han LI. Confirmed Diagnosis by RT-PCR and Phylogenetic Analysis of Peste des Petits Ruminants Viruses in Tibet, China[J]. Virologica Sinica, 2009, 24(6): 573-578. doi: 10.1007/s12250-009-3064-x
    Citation: Wen-hua ZHAO, Shi-biao YANG, Jian-qiang HAN, Mei JIANG, Hua-chun LI, Nian-zu ZHANG, Qi-han LI. Confirmed Diagnosis by RT-PCR and Phylogenetic Analysis of Peste des Petits Ruminants Viruses in Tibet, China .VIROLOGICA SINICA, 2009, 24(6) : 573-578.  http://dx.doi.org/10.1007/s12250-009-3064-x
    shu

    中国西藏小反刍兽疫病毒的RT-PCR确诊及遗传进化分析*

    • 1.

      ﹙1农业部热带亚热带动物病毒学重点开放实验室，国家外来动物疫病诊断实验室，云南昆明，650224; 2清华大学北京协和医学院，医学生物研究所，云南昆明，650118;3云南玉溪农业技术学院，云南玉溪，653100﹚

    • 通讯作者： 杨仕标, yangsb3799@sina.com; 
    • 收稿日期： 2009-04-21
      录用日期： 2009-07-06

    • 本研究对发生在2007年中国西藏的小反刍兽疫，采用巢式RT-PCR进行确诊并进行了遗传进化分析。研究结果显示：所检测11个样本均为PPR阳性，其中，2个测序样本同谱系I毒株Nigeria 75/1﹙X7443﹚同源性相近，3个样本同谱系IV毒株Sungri96﹙AY560591﹚具较近的同源关系；通过测定其中一株主要编码基因N/M/F/H全长读码框，发现其同毒株Sungri96的核苷酸序列同源性分别为96.6%、97.3%、97.6% 和98%。本研究结果显示中国西藏小反刍兽疫至少存在2种不同的传播来源。

    Confirmed Diagnosis by RT-PCR and Phylogenetic Analysis of Peste des Petits Ruminants Viruses in Tibet, China

    • 1.

      Laboratory of Tropical and Subtropical Animal Virology of MOA, National Exotic Animal Disease Diagnostic Laboratory, Kunming 650224, China

    • 2.

      Yu Xi Agriculture Vocational Technical College, Yu Xi 653100, China

    • 3.

      Peking Union Medical College, Tsinghua University, Kunming Jiaoling Road 379#, Kunming 650118, China

    • Corresponding author: Shi-biao YANG, yangsb3799@sina.com
    • Received Date: 21 April 2009
      Accepted Date: 06 July 2009

      Fund Project: Project from Yunnan Province 2008LA019project from MOA 2006-G57(3) B-Z1

    • This paper reports the confirmed diagnosis by nested RT-PCR of PPR cases in Tibet, China in 2007, and results of phylogenetic analysis. Results showed that the 11 tested samples were PPRV positive by nested RT-PCR, of which 2 samples were genetically close to the X7443 strain (Nigeria 75/1) of lineage I, and 3 samples close to the strain AY560591 (Sungri96) of linage IV with 96.6%、97.3%、97.6% and 98% nucleotide sequence homogeneity respectively, based on partial sequencing of the F gene from 5 samples and complete sequencing of the N/M/F/H genes from one sample. This study suggested that there are at least 2 origins of PPRV in China.
    • 加载中

      1. Ahmad M A, Nazmi O H, Nadim M A, et al. 2008. Seroprevalence of, and risk factors for, peste des petits ruminants in sheep and goats in Northern Jordan. Prev Veter Med, 85 (1-2): 1-8.
          doi: 10.1016/j.prevetmed.2008.01.002

      2. Bailey D, Banyard A, Dash P, et al. 2005. Full genome sequence of peste des petits ruminants virus, a member of the Morbillivirus genus. Virus Res, 110: 119-124.
          doi: 10.1016/j.virusres.2005.01.013

      3. Diallo A, Minet C, Le Goff C G, et al. 2007. The threat of peste des petits ruminants: progress in vaccine development for disease control. Vaccine, 25: 5591-5597.
          doi: 10.1016/j.vaccine.2007.02.013

      4. Food and farming group, International Trade Core Function. 2007. Peste des petits ruminants (PPR) in China. International Disease monitoring, Preminary outbreak assessment. Department for Environment Food and Rural Affairs, VITT 1200/BT-China, 27 July 2007. (http://www.defra.gov.uk/animalh/diseases/monitoring/pdf/ppr-china080714.pdf).

      5. Forsyth M A, Barrett T. 1995. Evaluation of polymerase chain reaction for the detection and characterization of rinderpest and peste des petits ruminants viruses for epidemiological studies. Virus Res, 39: 151-163.
          doi: 10.1016/0168-1702(95)00076-3

      6. Gargadennec L, Lalanne A. 1942. La peste des petits ruminants. Bulletin des Services Zoo Technoques et des Epizooties de I'Afrique Occidentale Francaise, 5: 16-21.

      7. Haffar A, Libeau G, Moussa A, et al.1999.The matrix protein gene sequence analysis reveals close relationship between peste des petits ruminants virus(PPRV)and dolphin morbillivirus. Virus Res, 64: 69-75.
          doi: 10.1016/S0168-1702(99)00080-5

      8. Jiang M, Yang S B, Zhang N Z. 2007. The epidemic trend and controlling of peste des petits ruminants(PPR). Prog Veter Med, 28 (supplement): 88-91. (in Chinese)

      9. Khan U Q M, Barrett T. 2008. Molecular based diagnosis of rinderpest and peste des petits ruminants virus in Pakistan. Int J Agri Biol, 10: 93-96.

      10. Kwiatek O, Minet C, Grillet C, et al. 2007. Peste des Petits Ruminants (PPR) Outbreak in Tajikistan. J Comp Path, 136: 111-119.
          doi: 10.1016/j.jcpa.2006.12.002

      11. Muthuchelvan D, Sanyal A, Sreenivasa B P, et al. 2006. Analysis of the matrix protein gene sequence of the Asian lineage of peste-des-petits ruminants vaccine virus. Vet Microbiol, 113: 83-87.
          doi: 10.1016/j.vetmic.2005.10.014

      12. Pronab D, Sreenivasa B P, Barrett T, et al. 2002. Recent epidemiology of peste des petits ruminants virus (PPRV). Vet Microbiol, 88: 153-159.
          doi: 10.1016/S0378-1135(02)00102-5

      13. Senthil C K, Dhinakar G R, Thangavelu A, et al. 2007. Performance of RT-PCR-ELISA for the detection of peste des petits ruminants virus. Small Ruminant Research, 72: 200-208.
          doi: 10.1016/j.smallrumres.2006.09.004

      14. Yang S B, Zhao W H, Jiang M. 2008. Study of serological survey on the Peste des petits ruminants. Chin Anim Heal, 11: 63-65. (in Chinese)

      15. Zhao W H, Yang S B, Jiang M, et al. 2007. Sequencing and analysis on the N、M、F and H genes of PPRV. Prog Veter Med, 28 (11): 8-11. (in Chinese)

      16. Zhao W H, Zhu J B, Yao L T, et al. 2002. Cloning and sequencing of HN gene of a goose paramyxovirus isolate. Chin J Veter Sci Technol, 32: 10-13. (in Chinese)

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      Confirmed Diagnosis by RT-PCR and Phylogenetic Analysis of Peste des Petits Ruminants Viruses in Tibet, China

        Corresponding author: Shi-biao YANG, yangsb3799@sina.com
      • 1. Laboratory of Tropical and Subtropical Animal Virology of MOA, National Exotic Animal Disease Diagnostic Laboratory, Kunming 650224, China
      • 2. Yu Xi Agriculture Vocational Technical College, Yu Xi 653100, China
      • 3. Peking Union Medical College, Tsinghua University, Kunming Jiaoling Road 379#, Kunming 650118, China
      • Received Date: 21 April 2009
      • Accepted Date: 06 July 2009
      Fund Project:  Project from Yunnan Province 2008LA019project from MOA 2006-G57(3) B-Z1

      Abstract: This paper reports the confirmed diagnosis by nested RT-PCR of PPR cases in Tibet, China in 2007, and results of phylogenetic analysis. Results showed that the 11 tested samples were PPRV positive by nested RT-PCR, of which 2 samples were genetically close to the X7443 strain (Nigeria 75/1) of lineage I, and 3 samples close to the strain AY560591 (Sungri96) of linage IV with 96.6%、97.3%、97.6% and 98% nucleotide sequence homogeneity respectively, based on partial sequencing of the F gene from 5 samples and complete sequencing of the N/M/F/H genes from one sample. This study suggested that there are at least 2 origins of PPRV in China.

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