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Volume 25 Issue 5
October 2010

    Yang-fei XIANG, Huai-qiang JU, Shen LI, Ying-jun ZHANG, Chong-ren YANG and Yi-fei WANG. Effects of 1, 2, 4, 6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg Secretion in HepG2.2.15 Cell Culture*[J]. Virologica Sinica, 2010, 25(5): 375-380. doi: 10.1007/s12250-010-3144-y
    Citation: Yang-fei XIANG, Huai-qiang JU, Shen LI, Ying-jun ZHANG, Chong-ren YANG, Yi-fei WANG. Effects of 1, 2, 4, 6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg Secretion in HepG2.2.15 Cell Culture* .VIROLOGICA SINICA, 2010, 25(5) : 375-380.  http://dx.doi.org/10.1007/s12250-010-3144-y
    shu

    余甘子多酚1, 2, 4, 6-四-没食子酰基-β-D –葡萄糖对HepG2.2.15细胞中HBsAg与HBeAg分泌的影响

    cstr: 32224.14.s12250-010-3144-y
    • 1.

      暨南大学生物医药研究开发基地，广东广州510632 2植物化学与西部植物资源持续利用国家重点实验室，中国科学院昆明植物研究所，云南昆明650204

    • 通讯作者： 王一飞, twang-yf@jnu.edu.cn
    • 收稿日期： 2010-04-14
      录用日期： 2010-06-07

    • 从中药余甘子（Phyllanthus emblica L.）中提取出多酚类化合物1, 2, 4, 6-四-没食子酰基-β-D –葡萄糖（1246TGG）并初步评价其抗乙型肝炎病毒（HBV）活性。细胞病变效应法（CPE）观察1246TGG对HepG2.2.25与HepG2细胞的毒性，酶联免疫吸附测定检测药物处理后HepG2.2.15细胞培养上清中HBsAg和HBeAg的分泌水平。结果表明HepG2.2.15细胞在无毒浓度的1246TGG（6.25 μg/mL、3.13 μg/mL）处理时HBsAg与HBeAg的分泌受到抑制，但随着检测时间的延长，抑制作用逐渐减弱。本研究为进一步评价1246TGG抗HBV活性及其作用机制提供了理论依据。

    Effects of 1, 2, 4, 6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg Secretion in HepG2.2.15 Cell Culture*

    • 1.

      Biomedicine Research & Development Center, Jinan University, Guangzhou 510630, China

    • 2.

      State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China

    • Corresponding author: Yi-fei WANG, twang-yf@jnu.edu.cn
    • Received Date: 14 April 2010
      Accepted Date: 07 June 2010

      Fund Project: Joint Funds of National Natural Science Foundation of China U0632010

    • A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG (6.25 μg/mL, 3.13 μg/mL), reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG.
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          doi: 10.1056/NEJM199712113372406

      8. Lee S J, Lee H K, Jung M K, et al. 2006. In vitro antiviral activity of 1, 2, 3, 4, 6-penta-O-galloyl-beta-D-glucose against hepatitis B virus. Biol Pharm Bull, 29 (10): 2131-2134.
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      10. Nakashima H, Ichiyama K, Hirayama F, et al. 1996. Sulfated pentagalloyl glucose (Y-ART-3) inhibits HIV replication and cytopathic effects in vitro, and reduces HIV infection in hu-PBL-SCID mice. Antiviral Res, 30 (2-3): 95-108.
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      11. Sun J, Hou J L. 2010. Management of chronic hepatitis B: experience from China. J Viral Hepatitis, 17 (S1): 10-17.

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      Effects of 1, 2, 4, 6-tetra-O-galloyl-β-D-glucose from P. emblica on HBsAg and HBeAg Secretion in HepG2.2.15 Cell Culture*

        Corresponding author: Yi-fei WANG, twang-yf@jnu.edu.cn
      • 1. Biomedicine Research & Development Center, Jinan University, Guangzhou 510630, China
      • 2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China
      • Received Date: 14 April 2010
      • Accepted Date: 07 June 2010
      Fund Project:  Joint Funds of National Natural Science Foundation of China U0632010

      Abstract: A polyphenolic compound, 1,2,4,6-tetra-O-galloyl-β-D-glucose (1246TGG), was isolated from the traditional Chinese medicine Phyllanthus emblica L. (Euphorbiaceae) and assayed for its potential as an anti-hepatitis B virus (HBV) agent. The cytotoxicity of 1246TGG on HepG2.2.15 as well as HepG2 cells was determined by observing cytopathic effects, and the effects of 1246TGG on secretion of HBsAg and HBeAg in HepG2.2.15 cells were assayed by enzyme immunoassay. Results indicates that treatment with 1246TGG (6.25 μg/mL, 3.13 μg/mL), reduced both HBsAg and HBeAg levels in culture supernatant, yet the inhibitory effects tend to decline with the assay time. This study provides a basis for further investigation of the anti-HBV activity and possible mechanism of action of 1246TGG.

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