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Volume 27 Issue 6
December 2012

    Raj Kishore Nanda, Bedaso Mammo Edao, Irshad Ahmad Hajam, Shanmugam Chandra Sekar, Kondabattula Ganesh, Veerakyathappa Bhanuprakash and Subodh Kishore. An Effective Mannosylated Chitosan Nanoparticle DNA Vaccine for FMD Virus[J]. Virologica Sinica, 2012, 27(6): 373-376. doi: 10.1007/s12250-012-3269-2
    Citation: Raj Kishore Nanda, Bedaso Mammo Edao, Irshad Ahmad Hajam, Shanmugam Chandra Sekar, Kondabattula Ganesh, Veerakyathappa Bhanuprakash, Subodh Kishore. An Effective Mannosylated Chitosan Nanoparticle DNA Vaccine for FMD Virus .VIROLOGICA SINICA, 2012, 27(6) : 373-376.  http://dx.doi.org/10.1007/s12250-012-3269-2
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    An Effective Mannosylated Chitosan Nanoparticle DNA Vaccine for FMD Virus

    cstr: 32224.14.s12250-012-3269-2

    • FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India

    • We are reporting you an effective DNA vaccine for FMD virus, and it was prepared using mannosylated chitosan (MC) nanoparticles to form the FMDV-pVAC-VP1-OmpA complex particles. These particles were characterized for their physical properties like morphology, size and charge prior evaluating the actual vaccine delivery effect of MC-nanoparticles. The immunological evaluation indicated that the 20 μg of DNA vaccine complexed with MC nano-particles was found optimum in inducing the immune response in G pigs as measured by FMDV specific neutralising antibodies and Th1/Th2 responses using micro SNT and ELISA, respectively.

    An Effective Mannosylated Chitosan Nanoparticle DNA Vaccine for FMD Virus

    • FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India

    • Corresponding author: Subodh Kishore, subodhkishore@gmail.com
    • Received Date: 05 July 2012
      Accepted Date: 07 November 2012
    • We are reporting you an effective DNA vaccine for FMD virus, and it was prepared using mannosylated chitosan (MC) nanoparticles to form the FMDV-pVAC-VP1-OmpA complex particles. These particles were characterized for their physical properties like morphology, size and charge prior evaluating the actual vaccine delivery effect of MC-nanoparticles. The immunological evaluation indicated that the 20 μg of DNA vaccine complexed with MC nano-particles was found optimum in inducing the immune response in G pigs as measured by FMDV specific neutralising antibodies and Th1/Th2 responses using micro SNT and ELISA, respectively.
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          doi: 10.1002/wnan.88

      2. Bozkir A, Saka O M. 2004. Chitosan nanoparticles for plasmid DNA delivery: effect of chitosan molecular structure on formulation and release characteristics. Drug Deliv, 11: 107-112.
          doi: 10.1080/10717540490280705

      3. Centelles M N, Qian C, Campanero M A, et al. 2008. New methodologies to characterize the effectiveness of the gene transfer mediated by DNA-chitosan nanoparticles. Int J Nanomed, 3(4): 451-460.

      4. Chinsangaram J, Beard C, Mason P W, et al. 1998. Antibody response in mice inoculated with DNA expressing foot-and-mouth disease virus capsid proteins. J Virol, 72: 4454-4457.

      5. Doel T R, 2003. FMD Vaccines. Virus Res, 91: 81-89.
          doi: 10.1016/S0168-1702(02)00261-7

      6. Hashimoto M, Morimoto M, Saimoto H, et al. 2006. Gene transfer by DNA/mannosylated chitosan complexes into mouse peritoneal macrophages. Biotechnol Lett, 28: 815-821.
          doi: 10.1007/s10529-006-9006-x

      7. Jang M K, Jeong Y I, Cho C S, et al. 2002. The preparation and characterization of low molecular and water soluble free-amine chitosan. Bull Korean Chem Soc, 23: 914-916.
          doi: 10.5012/bkcs.2002.23.6.914

      8. Jiang H L, Kang M L, Quan J S, et al. 2008. The potential of mannosylated chitosan microspheres to target macrophage mannose receptors in an adjuvant-delivery system for intranasal immunization, Biomaterials, 29: 1931-1939.
          doi: 10.1016/j.biomaterials.2007.12.025

      9. Jiang L, Qian F, He X, et al. 2007. Novel chitosan derivative nanoparticles enhance the immunogenicity of a DNA vaccine encoding hepatitis B virus core antigen in mice. J Gene Med, 9: 253-264.
          doi: 10.1002/(ISSN)1521-2254

      10. Kim T H, Jin H, Kim H W, et al. 2006. Mannosylated chitosan nanoparticle-based cytokine gene therapy suppressed cancer growth in BALB/c mice bearing CT-26 carcinoma cells. Mol Cancer Ther, 5: 1723-1732.
          doi: 10.1158/1535-7163.MCT-05-0540

      11. Lu S, Song X, Cao D, et al. 2004. Preparation of Water-Soluble Chitosan. J Appl Polymer Sci, 91: 3497-3503.
          doi: 10.1002/(ISSN)1097-4628

      12. McCullough K C, Sobrino F. 2004. Immunology of Foot and Mouth Disease. In: Foot and Mouth Disease current perspectives. Sobrino F, Domingo E, Ed. England: Horizon Bioscience, pp173-222.

      13. Mady M M, Darwish M M, Khalil S, et al. 2009. Biophysical studies on chitosan coated liposomes. Eur Biophysic J, 38: 1127-1133.
          doi: 10.1007/s00249-009-0524-z

      14. OIE Manual. 2008. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter No. 2. 1. 5. (Revised version May 2012), 6th Edition, Volume 1 and 2.

      15. Reed L J, Muench H A. 1938. A simple method of estimating 50% end points. Am J Hyg, 27: 493-497.

      16. Tan M C, Mommaas A M, Drijfhout J W, et al. 1997. Mannose receptor-mediated uptake of antigens strongly enhances HLA class Ⅱ-restricted antigen presentation by cultured dendritic cells. Eur J Immunol, 27: 2426-2435.
          doi: 10.1002/(ISSN)1521-4141

      17. Zhao F, Wu Y, Zhang X, et al.2011. Enhanced immune response and protective efficacy of a Treponema pallidum Tp92 DNA vaccine vectored by chitosan nanoparticles and adjuvanted with IL-2. Hum Vaccine. 1: 7(10).

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      An Effective Mannosylated Chitosan Nanoparticle DNA Vaccine for FMD Virus

        Corresponding author: Subodh Kishore, subodhkishore@gmail.com
      • FMD Quality Control and Quality Assurance Laboratory, Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore 560 024, Karnataka, India
      • Received Date: 05 July 2012
      • Accepted Date: 07 November 2012

      Abstract: We are reporting you an effective DNA vaccine for FMD virus, and it was prepared using mannosylated chitosan (MC) nanoparticles to form the FMDV-pVAC-VP1-OmpA complex particles. These particles were characterized for their physical properties like morphology, size and charge prior evaluating the actual vaccine delivery effect of MC-nanoparticles. The immunological evaluation indicated that the 20 μg of DNA vaccine complexed with MC nano-particles was found optimum in inducing the immune response in G pigs as measured by FMDV specific neutralising antibodies and Th1/Th2 responses using micro SNT and ELISA, respectively.

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