TRIM22 Inhibits the TRAF6-stimulated NF-κB Pathway by Targeting TAB2 for Degradation

Hui Qiu; Fang Huang; Han Xiao; Binlian Sun; Rongge Yang

doi:10.1007/s12250-013-3343-4

August 2013

Hui Qiu, Fang Huang, Han Xiao, Binlian Sun and Rongge Yang. TRIM22 Inhibits the TRAF6-stimulated NF-κB Pathway by Targeting TAB2 for Degradation[J]. Virologica Sinica, 2013, 28(4): 209-215. doi: 10.1007/s12250-013-3343-4

Citation: Hui Qiu, Fang Huang, Han Xiao, Binlian Sun, Rongge Yang. TRIM22 Inhibits the TRAF6-stimulated NF-κB Pathway by Targeting TAB2 for Degradation .VIROLOGICA SINICA, 2013, 28(4) : 209-215. http://dx.doi.org/10.1007/s12250-013-3343-4

TRIM22 Inhibits the TRAF6-stimulated NF-κB Pathway by Targeting TAB2 for Degradation

cstr: 32224.14.s12250-013-3343-4

Research Group of HIV Molecular Epidemiology and Virology, Center for Emerging Infectious Diseases, The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

通讯作者： Binlian Sun, sunbl@wh.iov.cn; Rongge Yang, ryang@wh.iov.cn
收稿日期： 2013-05-19
录用日期： 2013-05-27
出版日期： 2013-06-27

摘要

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines, but the detailed mechanisms governing this activation remains unclear. We investigated this mechanism in HEK293T cells. We found that overexpression of TRIM22 could activate the NF-κB pathway and conversely, could inhibit the tumor necrosis factor receptor-associated factor 6 (TRAF6)-stimulated NF-κB pathway in HEK293T cells. Further experiments showed that TRIM22 could decrease the self-ubiquitination of TRAF6, and interact with and degrade transforming growth factor-β activated kinase 1 binding protein 2 (TAB2), and that these effects could be partially rescued by a TRIM22 RING domain deletion mutant. Collectively, our data indicate that overexpression of TRIM22 may negatively regulate the TRAF6-stimulated NF-κB pathway by interacting with and degrading TAB2.
- TRIM22
- , NF-κB pathway
- , TRAF6
- , TAB2

TRIM22 Inhibits the TRAF6-stimulated NF-κB Pathway by Targeting TAB2 for Degradation

Research Group of HIV Molecular Epidemiology and Virology, Center for Emerging Infectious Diseases, The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

Corresponding author: Binlian Sun, sunbl@wh.iov.cn Rongge Yang, ryang@wh.iov.cn
Received Date: 19 May 2013
Accepted Date: 27 May 2013
Published Date: 27 June 2013

Abstract

Tripartite motif containing 22 (TRIM22), a member of the TRIM/RBCC family, has been reported to activate the nuclear factor-kappa B (NF-κB) pathway in unstimulated macrophage cell lines, but the detailed mechanisms governing this activation remains unclear. We investigated this mechanism in HEK293T cells. We found that overexpression of TRIM22 could activate the NF-κB pathway and conversely, could inhibit the tumor necrosis factor receptor-associated factor 6 (TRAF6)-stimulated NF-κB pathway in HEK293T cells. Further experiments showed that TRIM22 could decrease the self-ubiquitination of TRAF6, and interact with and degrade transforming growth factor-β activated kinase 1 binding protein 2 (TAB2), and that these effects could be partially rescued by a TRIM22 RING domain deletion mutant. Collectively, our data indicate that overexpression of TRIM22 may negatively regulate the TRAF6-stimulated NF-κB pathway by interacting with and degrading TAB2.
- TRIM22
- , NF-κB pathway
- , TRAF6
- , TAB2

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