Application of an M13 bacteriophage displaying tyrosine on the surface for detection of Fe<sup>3+</sup> and Fe<sup>2+</sup> ions

Xiaohua Guo; Chuncheng Niu; Yunhua Wu; Xiaosheng Liang

doi:10.1007/s12250-015-3651-y

December 2015

Xiaohua Guo, Chuncheng Niu, Yunhua Wu and Xiaosheng Liang. Application of an M13 bacteriophage displaying tyrosine on the surface for detection of Fe3+ and Fe2+ ions[J]. Virologica Sinica, 2015, 30(6): 410-416. doi: 10.1007/s12250-015-3651-y

Citation: Xiaohua Guo, Chuncheng Niu, Yunhua Wu, Xiaosheng Liang. Application of an M13 bacteriophage displaying tyrosine on the surface for detection of Fe³⁺ and Fe²⁺ ions .VIROLOGICA SINICA, 2015, 30(6) : 410-416. http://dx.doi.org/10.1007/s12250-015-3651-y

噬菌体表面酪氨酸展示用于铁及亚铁离子的检测

中南民族大学生命科学学院, 武汉 430074，中国

通讯作者： 梁晓声*, liangxs@mail.scuec.edu.cn, ORCID: 0000-0003-1482-6914
收稿日期： 2015-09-16
录用日期： 2015-12-03
出版日期： 2015-12-14

摘要

为了为发酵工厂建立一种不依赖仪器铁及亚铁离子检测方法，本研究建立了一种基于酪氨酸展示噬菌体的噬菌体传感技术。通过在噬菌体主要衣壳蛋白P8上插入一段含酪氨酸的短肽，得到了酪氨酸展示的噬菌体。酪氨酸上的酚羟基可与铁及亚铁离子发生特异性的螯合反应，因而设计酪氨酸展示噬菌体来探索其对铁离子检测的可能性。对该噬菌体的电子显微镜表征发现铁及亚铁离子可致噬菌体聚集，该聚集状态的噬菌体感染力下降。噬菌体感染力下降程度在一定范围内与铁及亚铁离子浓度成正比。本研究利用该噬菌体感染力与铁及亚铁离子浓度的关联进行了铁及亚铁离子的检测。对于铁离子，噬菌体的感染率相对下降量与铁离子浓度的对数在200 nM 到 8 μM线性相关，检测限为58 nM；对于亚铁离子，噬菌体的感染率相对下降量与铁离子浓度的对数在800 nM 到 8 μM线性相关，检测限为641.7 nM。本方法对铁及亚铁离子具有高度特异性，同等剂量的Ni2+, Pb2+, Zn2+, Mn2+, Co2+, Ca2+, Cu2+, Cr3+, Ba2+, 及 K+等离子无显著噬菌体感染力抑制效应。
- M13噬菌体
- , 展示
- , 酪氨酸
- , 铁离子
- , 亚铁离子
- , 聚集

Application of an M13 bacteriophage displaying tyrosine on the surface for detection of Fe³⁺ and Fe²⁺ ions

College of Life Science & Hubei Provincial Key Laboratory for Protection and Application of Special Plants on Wuling Area, South-Central University for Nationalities, Wuhan 430074, China

Corresponding author: Xiaosheng Liang, liangxs@mail.scuec.edu.cn
ORCID: 0000-0003-1482-6914
Received Date: 16 September 2015
Accepted Date: 03 December 2015
Published Date: 14 December 2015

Abstract

Ferric and ferrous ion plays critical roles in bioprocesses, their influences in many fields have not been fully explored due to the lack of methods for quantification of ferric and ferrous ions in biological system or complex matrix. In this study, an M13 bacteriophage (phage) was engineered for use as a sensor for ferric and ferrous ions via the display of a tyrosine residue on the P8 coat protein. The interaction between the specific phenol group of tyrosine and Fe3+ / Fe2+ was used as the sensor. Transmission electron microscopy showed aggregation of the tyrosine-displaying phages after incubation with Fe3+ and Fe2+. The aggregated phages infected the host bacterium inefficiently. This phenomenon could be utilized for detection of ferric and ferrous ions. For ferric ions, a calibration curve ranging from 200 nmol/L to 8 μmol/L with a detection limit of 58 nmol/L was acquired. For ferrous ions, a calibration curve ranging from 800 nmol/L to 8 μmol/L with a detection limit of 641.7 nmol/L was acquired. The assay was specific for Fe3+ and Fe2+ when tested against Ni2+, Pb2+, Zn2+, Mn2+, Co2+, Ca2+, Cu2+, Cr3+, Ba2+, and K+. The tyrosine displaying phage to Fe3+ and Fe2+ interaction would have plenty of room in application to biomaterials and bionanotechnology.
- M13 bacteriophage
- , tyrosine
- , display
- , ferric ion
- , ferrous ion
- , aggregation

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