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Volume 32 Issue 3
June 2017

    Yongxia Shi, Kui Zheng, Xiaobo Li, Liqiang Li, Shufen Li, Jinmin Ma, Jun Dai, Jingkai Ji, Shuai Yuan, Haorong Lu, Jiandong Li, Fangfang Sun, Xun Xu and Jicheng Huang. Isolation and phylogenetic study of Rift Valley fever virus from the first imported case to China[J]. Virologica Sinica, 2017, 32(3): 253-256. doi: 10.1007/s12250-017-3949-z
    Citation: Yongxia Shi, Kui Zheng, Xiaobo Li, Liqiang Li, Shufen Li, Jinmin Ma, Jun Dai, Jingkai Ji, Shuai Yuan, Haorong Lu, Jiandong Li, Fangfang Sun, Xun Xu, Jicheng Huang. Isolation and phylogenetic study of Rift Valley fever virus from the first imported case to China .VIROLOGICA SINICA, 2017, 32(3) : 253-256.  http://dx.doi.org/10.1007/s12250-017-3949-z
    shu

    中国首例输入性裂谷热病例的病毒分离与系统进化研究

    • 1.

      广东出入境检验检疫局检验检疫技术中心, 广州 510700, 中国

    • 2.

      深圳华大基因研究院, 深圳 518083, 中国

    • 3.

      哥本哈根大学生物学系, 哥本哈根, 丹麦

    • 4.

      中国科学院大学深圳华大研究中心, 深圳 518083, 中国

    • 5.

      深圳国家基因库, 深圳 518120, 中国

    • 通讯作者： 徐讯, xuxun@genomics.cn, ORCID: 0000-0002-5338-5173
      ; 黄吉城, jichenghuang@126.com, ORCID: 0000-0002-5882-7559
    • 收稿日期： 2017-01-22
      录用日期： 2017-04-12
      出版日期： 2017-05-02
    • 裂谷热病毒主要分布在非洲东部和南部地区，埃及、沙特阿拉伯和也门也报告过感染病例。近年来，进出口动物贸易的显著增加提高了裂谷热病毒传播的风险。该论文使用实时荧光RT-PCR检测、病毒分离、全基因组测序和系统进化分析等方法，对中国首例自安哥拉输入的裂谷热感染病例进行了分析。实时荧光RT-PCR结果显示该病例血清样品裂谷热病毒核酸阳性。接种后的Vero和BHK-21细胞中观察到典型的细胞病变效应，且在细胞培养上清中检测到裂谷热病毒核酸。对分离的BJ01裂谷热病毒进行全基因组深度测序，并将全基因组序列提交到GenBank， L、M和S基因片段登录号分别为KX632066、KX632067和KX632068。Blast分析表明所有三个基因片段与2009年南非的Kakamas羊分离株序列密切相关。L片段的DUF3770功能域N277S和S307N发生氨基酸突变，RDRP结构域三个氨基酸位点有突变，M片段NSm蛋白的V17I和G1蛋白的I385M位点发生非同义突变。系统进化分析显示，该病毒株位于进化树E分支，是目前E分支上第一个报道的可感染人类的裂谷热病毒，推测可能由于BJ01病毒株某些重要和关键氨基酸位点突变导致了从动物到人的跨种传播。

    Isolation and phylogenetic study of Rift Valley fever virus from the first imported case to China

    Isolation and phylogenetic study of Rift Valley fever virus

    • 1.

      Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510700, China

    • 2.

      BGI-Shenzhen, Shenzhen 518083, China

    • 3.

      Department of Biology, University of Copenhagen, Copenhagen 1017, Denmark

    • 4.

      BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China

    • 5.

      China National GeneBank-Shenzhen, BGI-Shenzhen, Shenzhen 518120, China#These authors contributed equally to this work.

    • Corresponding author: Xun Xu, xuxun@genomics.cn Jicheng Huang, jichenghuang@126.com
    • ORCID: 0000-0002-5338-5173; 0000-0002-5882-7559
    • Received Date: 22 January 2017
      Accepted Date: 12 April 2017
      Published Date: 02 May 2017
    • Here, laboratory detection, virus isolation, whole genome sequencing and phylogenetic analysis were performed to characterize the first imported case of Rift Valley fever virus infection returning from Angola.
    • 加载中

      1. Barzon L, Pacenti M, Franchin E, et al. 2014. J Clin Microbiol, 52: 3411–3413.
          doi: 10.1128/JCM.01328-14

      2. Bird BH, Khristova ML, Rollin PE, et al. 2007. J Virol, 81: 2805–2816.
          doi: 10.1128/JVI.02095-06

      3. Ikegami T. 2012. Antiviral Res, 95: 293–310.
          doi: 10.1016/j.antiviral.2012.06.001

      4. Ikegami T, Makino S. 2011. Viruses, 3: 493–519.
          doi: 10.3390/v3050493

      5. Jean Jose Nepomichene TN, Elissa N, Cardinale E, et al. 2015. J Med Entomol, 52: 962–969.
          doi: 10.1093/jme/tjv120

      6. LaBeaud AD, Cross PC, Getz WM, et al. 2011. Am J Trop Med Hyg, 84: 641–646.
          doi: 10.4269/ajtmh.2011.10-0187

      7. Liu W, Sun FJ, Tong YG, et al. 2016. Lancet Infect Dis, 16: 1226.

      8. Shivanna V, McDowell C, Wilson WC, et al. 2016. Genome Announc, 4. pii: e00926–16.

      9. Terasaki K, Won S, Makino S. 2013. J Virol, 87: 676–682.
          doi: 10.1128/JVI.02192-12

      10. Wood DE, Salzberg SL. 2014, Genome Biol, 15: R46.

      11. World Health Organization. 2016. Available: http://www.who.int/csr/don/02-august-2016-rift-valley-fever-china/en/. Accessed: 2 August 2016.

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      Isolation and phylogenetic study of Rift Valley fever virus from the first imported case to China

        Corresponding author: Xun Xu, xuxun@genomics.cn
        Corresponding author: Jicheng Huang, jichenghuang@126.com
      • 1. Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510700, China
      • 2. BGI-Shenzhen, Shenzhen 518083, China
      • 3. Department of Biology, University of Copenhagen, Copenhagen 1017, Denmark
      • 4. BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
      • 5. China National GeneBank-Shenzhen, BGI-Shenzhen, Shenzhen 518120, China#These authors contributed equally to this work.
      • Received Date: 22 January 2017
      • Accepted Date: 12 April 2017
      • Published Date: 02 May 2017

      Abstract: Here, laboratory detection, virus isolation, whole genome sequencing and phylogenetic analysis were performed to characterize the first imported case of Rift Valley fever virus infection returning from Angola.

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