The Functional Characterization of Bat and Human P[3] Rotavirus VP8*s

Dandi Li; Mengxuan Wang; Tongyao Mao; Mingwen Wang; Qing Zhang; Hong Wang; Lili Pang; Xiaoman Sun; Zhaojun Duan

doi:10.1007/s12250-021-00400-z

October 2021

Dandi Li, Mengxuan Wang, Tongyao Mao, Mingwen Wang, Qing Zhang, Hong Wang, Lili Pang, Xiaoman Sun and Zhaojun Duan. The Functional Characterization of Bat and Human P[3] Rotavirus VP8*s[J]. Virologica Sinica, 2021, 36(5): 1187-1196. doi: 10.1007/s12250-021-00400-z

Citation: Dandi Li, Mengxuan Wang, Tongyao Mao, Mingwen Wang, Qing Zhang, Hong Wang, Lili Pang, Xiaoman Sun, Zhaojun Duan. The Functional Characterization of Bat and Human P[3] Rotavirus VP8*s .VIROLOGICA SINICA, 2021, 36(5) : 1187-1196. http://dx.doi.org/10.1007/s12250-021-00400-z

蝙蝠和人P[3]轮状病毒VP8*蛋白的功能研究

李丹地 ^1,2, ,
王萌璇 ^1,2 ,
毛彤瑶 ^1,2 ,
王明雯 ^1,2, ,
章青 ^1,2 ,
王宏 ^1,2 ,
庞立丽 ^1,2 ,
孙晓曼 ^1,2,, ,
段招军 ^1,2,,

cstr: 32224.14.s12250-021-00400-z

2.
中国疾病预防控制中心病毒病预防控制所

通讯作者： 孙晓曼, sunxiaoman88@163.com, ORCID: http://orcid.org/0000-0002-2601-8239
; 段招军, zhaojund@126.com, ORCID: http://orcid.org/0000-0003-3090-4150
收稿日期： 2021-01-06
录用日期： 2021-04-12
出版日期： 2021-05-31

摘要

P[3]型轮状病毒在许多物种中都有发现，包括人、猴、狗和蝙蝠等。关于轮状病毒的受体已有很多研究，据报导多种聚糖可以作为轮状病毒进入宿主细胞的粘附分子，包括唾液酸、组织血型抗原（HBGAs）。本研究对不同物种P[3]型轮状病毒VP8*s的多糖结合特性进行探索。研究发现人HCR3A和狗P[3]型轮状病毒VP8*结合唾液酸以及末端带有唾液酸的聚糖。，同时，人和狗P[3]型轮状病毒VP8*还可以凝集不同物种红细胞，而蝙蝠P[3]型轮状病毒VP8*既不与聚糖结合，也不发生血凝。进一步研究显示蝙蝠P[3]型轮状病毒P[3] VP8* C189Y突变体可以凝集红细胞，而人P[3] HCR3A/M2-102 Y189C突变体则不再凝集红细胞。序列比对和结构分析表明，VP8*蛋白189位氨基酸在轮状病毒VP8*配体识别中起着重要作用，氨基酸的改变可能促进轮状病毒跨物种传播。结构比对显示蝙蝠P[3]型 VP8*的模拟结构与RRV 猴P[3]型VP8*有很大差异，尤其糖结合位点区域构象也呈现不同特征，表明蝙蝠P[3]型轮状病毒相对与其他P[3]型轮状病毒是不同的明显不同，一定程度上解释了蝙蝠P[3]轮状病毒VP8*不结合唾液酸多糖。这些研究促进了我们对P[3]型轮状病毒VP8*与多糖相互作用以及蝙蝠和人P[3]轮状病毒传播的认识，为探索轮状病毒的感染和流行提供了更多的信息。
- 蝙蝠轮状病毒
- , P[3] VP8*
- , 糖结合特性
- , 血凝
- , 唾液酸

The Functional Characterization of Bat and Human P[3] Rotavirus VP8*s

Dandi Li ^1,2, ,
Mengxuan Wang ^1,2 ,
Tongyao Mao ^1,2 ,
Mingwen Wang ^1,2, ,
Qing Zhang ^1,2 ,
Hong Wang ^1,2 ,
Lili Pang ^1,2 ,
Xiaoman Sun ^1,2,, ,
Zhaojun Duan ^1,2,,

1.
National Health Commission Key Laboratory for Medical Virology and Viral Diseases, Beijing, 102206, China
2.
National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 102206, China

Corresponding author: Xiaoman Sun, sunxiaoman88@163.com Zhaojun Duan, zhaojund@126.com
ORCID: http://orcid.org/0000-0002-2601-8239; http://orcid.org/0000-0003-3090-4150
Received Date: 06 January 2021
Accepted Date: 12 April 2021
Published Date: 31 May 2021

Abstract

P[3] rotavirus (RV) has been identified in many species, including human, simian, dog, and bat. Several glycans, including sialic acid, histo-blood group antigens (HBGAs) are reported as RV attachment factors. The glycan binding specificity of different P[3] RV VP8*s were investigated in this study. Human HCR3A and dog P[3] RV VP8*s recognized glycans with terminal sialic acid and hemagglutinated the red blood cells, while bat P[3] VP8* showed neither binding to glycans nor hemagglutination. However, the bat P[3] VP8* mutant of C189Y obtained the ability to hemagglutinate the red blood cells, while human P[3] HCR3A/M2-102 mutants of Y189C lost the ability. Sequence alignment and structural analysis indicated that residue 189 played an important role in the ligand recognition and may contribute to the cross-species transmission. Structural superimposition exhibited that bat P[3] VP8* model was quite different from the simian P[3] Rhesus rotavirus (RRV) P[3] VP8*, indicating that bat P[3] RV was relatively distinct and partially contributed to the no binding to tested glycans. These results promote our understanding of P[3] VP8*/glycans interactions and the potential transmission of bat/human P[3] RVs, offering more insight into the RV infection and prevalence.
- Bat rotavirus
- , P[3] VP8*
- , Glycan binding specificity
- , Hemagglutination
- , Sialic acid

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