Identification and genome characterization of circovirus in masked palm civets (<em>Paguma larvata</em>) from Guangdong Province, China

Pian Zhang; Jing Chen; Xiaofan Chen; Chenglong Sun; Zhaowen Ren; Yin Mei; Fumin Wang; Xiaoxiao Zhang; Yangrui Lu; Xu Wang; Hua Xiang; Yuan Huang; Gang Wang; Ming Liao; Xiaohu Wang

doi:10.1016/j.virs.2026.09.001

October 2026

. doi: 10.1016/j.virs.2026.09.001

Citation: Pian Zhang, Jing Chen, Xiaofan Chen, Chenglong Sun, Zhaowen Ren, Yin Mei, Fumin Wang, Xiaoxiao Zhang, Yangrui Lu, Xu Wang, Hua Xiang, Yuan Huang, Gang Wang, Ming Liao, Xiaohu Wang. Identification and genome characterization of circovirus in masked palm civets (Paguma larvata) from Guangdong Province, China .VIROLOGICA SINICA, 2026, 41(5) : 1247-1250. http://dx.doi.org/10.1016/j.virs.2026.09.001

广东省果子狸圆环病毒的鉴定及基因组特征分析

张翩 ^a ,
陈晶 ^a ,
陈晓凡 ^a ,
孙成龙 ^b ,
任照文 ^a ,
梅隐 ^c ,
王付民 ^c ,
张晓晓 ^a ,
卢杨睿 ^a ,
王旭 ^a,d ,
向华 ^a ,
黄元 ^a ,
王刚 ^a,, ,
廖明 ^a,d,, ,
王晓虎 ^a,,

a. 广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室/广东省动物疫病野外科学观测站;
b. 美国德克萨斯大学西南医学中心分子生物学系;
c. 广东省野生动物监测救护中心;
d. 仲恺农业工程学院动物科技学院

通讯作者： 王刚, wanggang@gdaas.cn; 廖明, mliao@scau.edu.cn; 王晓虎, wangxiaohu2020@163.com
收稿日期： 2026-03-27
录用日期： 2026-09-01

摘要

果子狸是我国南方地区重要的人工繁育野生动物，其携带病毒的多样性及潜在跨物种传播风险值得关注。本研究对2020年采自广东省5个城市的103只人工繁育果子狸共173份样本开展病毒宏基因组测序和PCR检测，首次在中国大陆报道了果子狸源圆环病毒（Pl-CV）的完整基因组序列。结果显示，Pl-CV DNA个体检出率为34.0%（35/103）；在韶关某养殖场采集的35只果子狸中，肝脏和肠道样本的检出率分别为31.4%和25.7%，胃组织中未检出。研究共获得4条Pl-CV完整基因组序列，基因组全长为2,044～2,067 nt，均具有圆环病毒典型的Rep和Cap开放阅读框、茎环结构及滚环复制相关保守基序。系统发育分析表明，4株病毒与日本报道的果子狸源圆环病毒聚为独立分支，完整基因组核苷酸相似性为87.4%～90.0%。本研究扩展了Pl-CV的已知地理分布，为认识人工繁育果子狸携带圆环病毒的遗传特征及开展野生动物病原监测提供了基础资料。
- 果子狸
- , 圆环病毒
- , 病毒宏基因组
- , 全基因组
- , 系统发育分析

Identification and genome characterization of circovirus in masked palm civets (Paguma larvata) from Guangdong Province, China

Pian Zhang ^a ,
Jing Chen ^a ,
Xiaofan Chen ^a ,
Chenglong Sun ^b ,
Zhaowen Ren ^a ,
Yin Mei ^c ,
Fumin Wang ^c ,
Xiaoxiao Zhang ^a ,
Yangrui Lu ^a ,
Xu Wang ^a,d ,
Hua Xiang ^a ,
Yuan Huang ^a ,
Gang Wang ^a,, ,
Ming Liao ^a,d,, ,
Xiaohu Wang ^a,,

a. Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
b. Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA;
c. Guangdong Provincial Wildlife Monitoring and Rescue Center, Guangzhou 510000, China;
d. College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

Corresponding author: Gang Wang, wanggang@gdaas.cn Ming Liao, mliao@scau.edu.cn Xiaohu Wang, wangxiaohu2020@163.com
Received Date: 27 March 2026
Accepted Date: 01 September 2026

Abstract

Highlights
1 First identification of Pl-CV in Chinese mainland, with a 34.0% detection rate in farmed civets.
2 Pl-CV DNA was detected in liver (31.4%) and intestine (25.7%) specimens from one Shaoguan farm.
3 Four complete genomes show 87.4%–90.0% nucleotide identity with Japanese civet circoviruses.

References
1. Breitbart, M., Delwart, E., Rosario, K., Segales, J., Varsani, A., ICTV Report Consortium, 2017. ICTV Virus Taxonomy Profile: Circoviridae. J. Gen. Virol. 98, 1997-1998.
2. Chen, S., Li, W., Fan, L., Xu, C., Liu, S., Li, H., Liu, P., Zhu, W., Wu, X., Qin, P., Li, J., Ma, X., Wei, Y., 2026. Metatranscriptomics profiling reveals rodent- and shrew-borne viral diversity and evolutionary relationships in Guangzhou, China. Virol. Sin. 41, 35-47.
3. Dai, Z., Wang, H., Feng, Z., Ma, L., Yang, S., Shen, Q., Wang, X., Zhou, T., Zhang, W., 2021. Identification of a novel circovirus in blood sample of giant pandas (Ailuropoda melanoleuca). Infect. Genet. Evol. 95, 105077.
4. Delwart, E., Li, L., 2012. Rapidly expanding genetic diversity and host range of the Circoviridae viral family and other Rep encoding small circular ssDNA genomes. Virus Res. 164, 114-121.
5. Dowgier, G., Lorusso, E., Decaro, N., Desario, C., Mari, V., Lucente, M.S., Lanave, G., Buonavoglia, C., Elia, G., 2017. A molecular survey for selected viral enteropathogens revealed a limited role of Canine circovirus in the development of canine acute gastroenteritis. Vet. Microbiol. 204, 54-58.
6. Kalyaanamoorthy, S., Minh, B.Q., Wong, T.K.F., von Haeseler, A., Jermiin, L.S., 2017. ModelFinder: fast model selection for accurate phylogenetic estimates. Nat. Methods. 14, 587-589.
7. Mankertz, A., Hillenbrand, B., 2001. Replication of porcine circovirus type 1 requires two proteins encoded by the viral rep gene. Virology 279, 429-438.
8. Nishizawa, T., Sugimoto, Y., Takeda, T., Kodera, Y., Hatano, Y., Takahashi, M., Okamoto, H., 2018. Identification and full-genome characterization of novel circoviruses in masked palm civets (Paguma larvata). Virus Res. 258, 50-54.
9. Wang, L.F., Eaton, B.T., 2007. Bats, civets and the emergence of SARS. Curr. Top. Microbiol. Immunol. 315, 325-344.
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10.1016j.virs.2026.09.001-ESM.docx

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Identification and genome characterization of circovirus in masked palm civets (Paguma larvata) from Guangdong Province, China

Corresponding author: Gang Wang, wanggang@gdaas.cn

Corresponding author: Ming Liao, mliao@scau.edu.cn

Corresponding author: Xiaohu Wang, wangxiaohu2020@163.com

a. Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Guangdong Provincial Observation and Research Station for Animal Disease, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;

b. Department of Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA;

c. Guangdong Provincial Wildlife Monitoring and Rescue Center, Guangzhou 510000, China;

d. College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

Received Date: 27 March 2026

Accepted Date: 01 September 2026

Abstract: Highlights
1 First identification of Pl-CV in Chinese mainland, with a 34.0% detection rate in farmed civets.
2 Pl-CV DNA was detected in liver (31.4%) and intestine (25.7%) specimens from one Shaoguan farm.
3 Four complete genomes show 87.4%–90.0% nucleotide identity with Japanese civet circoviruses.

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广东省果子狸圆环病毒的鉴定及基因组特征分析

摘要

Identification and genome characterization of circovirus in masked palm civets (Paguma larvata) from Guangdong Province, China

Abstract

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通讯作者: 陈斌, bchen63@163.com

Identification and genome characterization of circovirus in masked palm civets (Paguma larvata) from Guangdong Province, China

Corresponding author: Gang Wang, wanggang@gdaas.cn

Corresponding author: Ming Liao, mliao@scau.edu.cn

Corresponding author: Xiaohu Wang, wangxiaohu2020@163.com

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