Sequence Analysis of the 1ef-3 Gene of Helicoverpa armigera Single-nucleocapsid Nucle0pOlyhedr0virus

ZHANG Xiao—xia; LIANG Zhen—pu; NIU Guo—dong; ZHANG Zhong—xin ．DING Qing—quan

doi:1003—5125(2002)03—0234—05

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Volume 17 Issue 3

June 2002

ZHANG Xiao—xia, LIANG Zhen—pu, NIU Guo—dong, ZHANG Zhong—xin ．DING Qing—quan and Sequence Analysis of the 1ef-3 Gene of Helicoverpa armigera Single-nucleocapsid Nucle0pOlyhedr0virus[J]. Virologica Sinica, 2002, 17(3): 234-238. doi: 1003—5125(2002)03—0234—05

Citation: ZHANG Xiao—xia, LIANG Zhen—pu, NIU Guo—dong, ZHANG Zhong—xin ．DING Qing—quan, . Sequence Analysis of the 1ef-3 Gene of Helicoverpa armigera Single-nucleocapsid Nucle0pOlyhedr0virus .VIROLOGICA SINICA, 2002, 17(3) : 234-238. http://dx.doi.org/1003—5125(2002)03—0234—05

棉铃虫核多角体病毒z 一3基因的序列分析

1.
中国科学院武汉病毒研究所．湖北武汉430071

摘要

在棉铃虫核多角体病毒(Helicoverpa armigera Single-nucleocapsid Nucleapolyhedrovirus，HaSNPV)基因组中发现了．3基因，该基因位于病毒基因组的BarnHI F片段上，全长1140bp，编码379个氨基酸，预计蛋白质分子量为44kD．启动子区具有TATAbox，位于起始密码子ATG上游40～43nt处，在终止密码子下游具典型的poly(A)终止信号AATAAA。并且在它的氨基酸序列的一端也具有一个类似SSB保守序列的基元结构。
- lef-3
- , 序列分析
- , 基元结构
- , SSB

Sequence Analysis of the 1ef-3 Gene of Helicoverpa armigera Single-nucleocapsid Nucle0pOlyhedr0virus

1.
Wuhan Institute of Virology ，Chinese Academy ofSciences，Wuhan 430071，China

Abstract

An lef一3 gene of HaSNPV was identified．It was 1ocalized in Bam H 工一F fragment of the genome．Nucleotide sequencing showed the open reading frame was 1 1 40 nt，encoded 379 amino acids with a predicted size of 44kD．A ”TATA”box was found in 40～ 43nt upstream of the transcriptiona1 start codon ATG．A typical poly(A)signal，AATAAA，was found in the downstream of the tran— scriptional stop codon．HaSPV LEF一3 protein also contains the conserved motif。which exists in nearly 20 kinds of SSBs，SO HaSNPV LEF一3 protein iS a probable SSB protein
- Zef一3
- , Sequence analysis
- , Motif SSB

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Sequence Analysis of the 1ef-3 Gene of Helicoverpa armigera Single-nucleocapsid Nucle0pOlyhedr0virus

1. Wuhan Institute of Virology ，Chinese Academy ofSciences，Wuhan 430071，China

Abstract: An lef一3 gene of HaSNPV was identified．It was 1ocalized in Bam H 工一F fragment of the genome．Nucleotide sequencing showed the open reading frame was 1 1 40 nt，encoded 379 amino acids with a predicted size of 44kD．A ”TATA”box was found in 40～ 43nt upstream of the transcriptiona1 start codon ATG．A typical poly(A)signal，AATAAA，was found in the downstream of the tran— scriptional stop codon．HaSPV LEF一3 protein also contains the conserved motif。which exists in nearly 20 kinds of SSBs，SO HaSNPV LEF一3 protein iS a probable SSB protein