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Volume 22 Issue 1
February 2007

    Xue-mei ZHANG, Yan-chun CHE, Jing-jing WANG, Long-ding LIU, Ming-xue XIE and Qi-han LI. Complete Genomic Sequence Analysis of a New SV40 Isolate[J]. Virologica Sinica, 2007, 22(1): 41-45.
    Citation: Xue-mei ZHANG, Yan-chun CHE, Jing-jing WANG, Long-ding LIU, Ming-xue XIE, Qi-han LI. Complete Genomic Sequence Analysis of a New SV40 Isolate .VIROLOGICA SINICA, 2007, 22(1) : 41-45.
    shu

    一株新的SV40分离株—— SV-IMB的全基因组序列分析

    • 中国医学科学院 中国协和医科大学 医学生物学研究所病毒免疫室 昆明 650118

    • 通讯作者： 李琦涵*, qihanlister@gmail.com
    • 收稿日期： 2006-08-14
      录用日期： 2006-11-20
    • 摘要：我们对分离自恒河猴指名亚种的一株SV40——SV-IMB进行了全基因组序列测定，并与其它分离株进行了比较分析。结果表明：SV-IMB基因组全长5246bp，与其它毒株的平均同源性为98.1% 。调控区具有一个完整的增强子和一个缺损的增强子。大T抗原和VP1都发生了不同程度的氨基酸变异。结果表明这是一株新的SV40分离株。

    Complete Genomic Sequence Analysis of a New SV40 Isolate

    • Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming,Yunnan Province,650118,China

    • Corresponding author: Qi-han LI, qihanlister@gmail.com
    • Received Date: 14 August 2006
      Accepted Date: 20 November 2006

      Fund Project: National Natural Science Founds 30570081

    • The genome of a new SV40 strain（SV-IMB）isolated from a rhesus monkey was completely sequenced and compared with other isolates. The results showed that the whole genome contains 5246bp, and the average identity of SV-IMB was 98.1% as compared to other SV40 isolates. Its regulatory region consists of a complete enhancer and a defective enhancer. Amino acid changes occurred to some extent in both the large T antigen (T-Ag) and VP1 region. Our findings demonstrate that the SV-IMB is a new SV40 isolate.
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          doi: 10.1038/sj.onc.1209046

      2. Dang X,Axthelm M K,Letvin N L,et al. 2005. Rearrangement of simian virus 40 regulatory region is not required for induction of progressive multifocal leukoencephalopathy in immunosuppressed rhesus monkeys[J]. J Virol,79(3): 1361-1366.
          doi: 10.1128/JVI.79.3.1361-1366.2005

      3. Eddy B E,Borman G S,Grubbs G E,et al. 1962. Identification of the oncogenic substance in rhesus monkey kidney cell culture as simian virus 40[J]. Virology,17:65-75.
          doi: 10.1016/0042-6822(62)90082-X

      4. Forsman Z H,Lednicky J A,Fox G E,et al. 2004. Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation[J]. J Virol,78(17): 9306-9316.
          doi: 10.1128/JVI.78.17.9306-9316.2004

      5. Galateau-Salle F,Bidet P,Iwatsubo Y,et al. 1998. Detection of SV40-like DNA sequences in pleural mesothelioma,bronchopulmonary carcinoma and other pulmonary diseases[J]. Dev Biol Stand,94:147-152.

      6. Geissler E,Staneczek W. 1988. SV40 and human brain tu-mors[J]. Arch Geschwulstforsch,58(2): 129-134.

      7. Lednicky J A,Arrington A S,Stewart A R,et al. 1998. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease[J]. J Virol,72(5): 3980-3990.

      8. Scheidtmann K H,Buck M,Schneider J,et al. 1991. Biochemical characterization of phosphorylation site mutants of simian virus 40 large T antigen: evidence for interaction between amino- and carboxy-terminal domains[J]. J Virol,65(3): 1479-1490.

      9. Shah K V,Galloway D A,Knowles W A,et al. 2004. Simian virus 40 (SV40) and human cancer: a review of the serolo-gical data[J]. Rev Med Virol,14(4): 231-239.
          doi: 10.1002/(ISSN)1099-1654

      10. Sullivan C S,Pipas J M. 2002. T antigens of simian virus 40: molecular chaperones for viral replication and tumorigenesis[J]. Microbiol Mol Biol Rev,66(2): 179-202.
          doi: 10.1128/MMBR.66.2.179-202.2002

      11. Sweet B H,Hilleman M R. 1960. The vacuolating virus,S.V. 40[J]. Proc Soc Exp Biol Med,105: 420-427.
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      12. Weiner L P,Herndon R M,Narayan O,et al. 1972. Isolation of virus related to SV40 from patients with progressive multifocal leukoencephalopathy[J]. N Engl J Med,286(8): 385-390.
          doi: 10.1056/NEJM197202242860801

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      Complete Genomic Sequence Analysis of a New SV40 Isolate

        Corresponding author: Qi-han LI, qihanlister@gmail.com
      • Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming,Yunnan Province,650118,China
      • Received Date: 14 August 2006
      • Accepted Date: 20 November 2006
      Fund Project:  National Natural Science Founds 30570081

      Abstract: The genome of a new SV40 strain（SV-IMB）isolated from a rhesus monkey was completely sequenced and compared with other isolates. The results showed that the whole genome contains 5246bp, and the average identity of SV-IMB was 98.1% as compared to other SV40 isolates. Its regulatory region consists of a complete enhancer and a defective enhancer. Amino acid changes occurred to some extent in both the large T antigen (T-Ag) and VP1 region. Our findings demonstrate that the SV-IMB is a new SV40 isolate.

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