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Volume 22 Issue 1
February 2007

    Sheng-Li MENG, Ge-Lin XU, Jia-Xin YAN, Ping-Gang MING, Jie WU, Xiao-Ming YANG, He-Tian MING, Feng-Cai ZHU, Dun-Jin ZHOU, QI-You XIAO and Guan-Mu DONG. Molecular Epidemiology and Sequencing of the G-L Intergenic Region of RabiesViruses Isolated in China[J]. Virologica Sinica, 2007, 22(1): 26-33.
    Citation: Sheng-Li MENG, Ge-Lin XU, Jia-Xin YAN, Ping-Gang MING, Jie WU, Xiao-Ming YANG, He-Tian MING, Feng-Cai ZHU, Dun-Jin ZHOU, QI-You XIAO, Guan-Mu DONG. Molecular Epidemiology and Sequencing of the G-L Intergenic Region of RabiesViruses Isolated in China .VIROLOGICA SINICA, 2007, 22(1) : 26-33.
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    中国分离的狂犬病毒G-L基因间隔区的序列测定与分子流行病学

    • 1.

      武汉生物制品研究所 武汉 430060;2 阜阳市疾病控制中心 安徽阜阳 ;3 江苏省疾病控制中心 南京;4武汉市疾病控制中心 武汉;5 湖南省疾病控制中心 长沙;6中国药品生物制品检定所　北京

    • 通讯作者： 严家新, yanjx45@163.com; 
    • 收稿日期： 2006-07-31
      录用日期： 2006-08-31
    • 摘要：在2004－2006年期间，从中国不同地区共收集到25株狂犬病毒，其中24株是从犬脑分离，1株是从人脑分离。对这25株街毒株和7个代次的CTN疫苗株的G-L基因间隔区进行了序列测定和系统进化关系分析。结果显示：所有中国毒株的G-L基因间隔区都由519个碱基构成，这25株街毒株之间核苷酸序列同源性为95.5％-100%。本文所分析的所有中国毒株都属于基因 1型，其详细分布情况与地理上的来源相关。这些毒株共可分为密切相关的两个类群，所有街毒株为一个类群，不同代次的CTN疫苗株为另一个类群。此项研究提供了狂犬病毒基于G－L基因间隔区序列的分子流行病学详细资料。

    Molecular Epidemiology and Sequencing of the G-L Intergenic Region of RabiesViruses Isolated in China

    • 1.

      Wuhan Institute of Biological Products,Wuhan 430060,China

    • 2.

      Fuyang Center for Disease Control and Prevention,Fuyang 236000,China

    • 3.

      Jiangsu Center for Disease Control and Prevention,Nanjing 210009 China

    • 4.

      Wuhan Center for Disease Control and Prevention,Wuhan 430015,China

    • 5.

      Hunan Center for Disease Control and Prevention,Changsha 410005,China

    • 6.

      National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China

    • Corresponding author: Jia-Xin YAN, yanjx45@163.com
    • Received Date: 31 July 2006
      Accepted Date: 31 August 2006

      Fund Project: The Key Technologies R & D Programme of the 10th National five-year-plan 2004BA718 b03

    • A group of 25 rabies viruses (RABVs), recovered from 24 dogs and one human case, were collected from various areas in China between 2004 and 2006. Genetic and phylogenetic analyses of the G-L intergenic region were carried out in 25 street RABV isolates and CTN vaccine strains of 7 generations. The study was based on the comparison of a 519 bp nucleotide sequence, encompassing the G-L intergenic region. The nucleotide sequence homologies of Chinese street strains were from 95.5% to 100%. The phylogenetic analysis showed that all Chinese isolates clearly supported the placement of all Chinese viruses in Lyssavirus genotype 1 and they were distributed according to their geographical origins. All of the Chinese strains were closely related but they could still be divided into two groups: group of street strains and group of CTN strains. This study presents details about the molecular epidemiology of rabies viruses based on the sequences of the G-L Intergenic region.
    • 加载中

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          doi: 10.1128/JVI.75.7.3268-3276.2001

      2. Dodet B,Meslin F-X,Aubert. 2001. In: Rabies Control in Asia[M]. Paris. John Libbey Eurotext,191-196.

      3. Hillis D M,Bull J J. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis[J]. Systematic Biol,42: 182-192
          doi: 10.1093/sysbio/42.2.182

      4. Kimura M. 2004. A simple method for estimating evolutionary rates of base substitutions through compara-tive studies of nucleotide sequences[J]. Briefings in Bioinformatics,5: 150-163
          doi: 10.1093/bib/5.2.150

      5. Kumar S,Tamura K,Nei M. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment[J]. Brief Bioinform,5(2): 150-163.
          doi: 10.1093/bib/5.2.150

      6. Kuzmin I V,Hughes G J,Botvinkin A D,et al. 2005. Phylogenetic relationships of Irkut and West Caucasian bat viruses within the Lyssavirus genus and suggested quantitative criteria based on the N gene sequence for lyssavirus genotype definition[J]. Virus Res,111(1): 28-43.
          doi: 10.1016/j.virusres.2005.03.008

      7. Meslin F X,Kaplan M M,Koprowski H,et al. 1996. Labo-ratory techniques in rabies[M]. 4th ed. Geneva. World Health Organization

      8. Nadin-Davis S A,Abdel-Malik M,Armstrong J,et al. 2002. Lyssavirus P gene characterisation provides insights into the phylogeny of the genus and identifies structural similarities and diversity within the encoded phosphoprotein[J]. Virology,298(2): 286-305.
          doi: 10.1006/viro.2002.1492

      9. Nel L H,Sabeta C T,von Teichman B,et al. 2005. Mongoose rabies in southern Africa: a re-evaluation based on mo-lecular epidemiology[J]. Virus Res,109(2): 165-173.
          doi: 10.1016/j.virusres.2004.12.003

      10. Ravkov E V,Smith J S,Nichol S T. 1995. Rabies virus glycoprotein gene contains a long 3' noncoding region which lacks pseudogene properties[J]. Virology,206(1): 718-723.
          doi: 10.1016/S0042-6822(95)80095-6

      11. Sato G,Itou T,Shoji Y,et al. 2004. Genetic and phylogenetic analysis of glycoprotein of rabies virus isolated from several species in Brazil[J]. J Vet Med Sci,66(7): 747-753.
          doi: 10.1292/jvms.66.747

      12. Thompson J D,Higgins D G,Gibson T. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting,position-specific gap penalties and weight matrix choice [J]. Nucleic Acids Res,22: 4673-4680
          doi: 10.1093/nar/22.22.4673

      13. Velazquez-Monroy O,Vargas-Pino F,Gutierrez-Cedillo V,et al. 2003. Advances in canine rabies control in Mexico[D]. The XVI international conference-Rabies in the Americas Philadelphia: Thomas Jefferson University. p78.

      14. WHO. 2005. Expert Consultation on rabies[J]. World Health Organ Tech Rep Ser,931: 1-88.

      15. Xu G L,Ku L,Wu J,et al. 2002. Sequence analysis of N Gene among 19 rabies virus street strains from China[J]. Vi-rologica Sinica,18: 48-51.(in Chinese)

      16. Zhang Y Z,Xiong C L,Zou Y,et al. 2006. Molecular cha-racterization of rabies virus isolates in China during 2004[J]. Virus Res,121(2): 179-188.
          doi: 10.1016/j.virusres.2006.05.010

    • 加载中

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      Molecular Epidemiology and Sequencing of the G-L Intergenic Region of RabiesViruses Isolated in China

        Corresponding author: Jia-Xin YAN, yanjx45@163.com
      • 1. Wuhan Institute of Biological Products,Wuhan 430060,China
      • 2. Fuyang Center for Disease Control and Prevention,Fuyang 236000,China
      • 3. Jiangsu Center for Disease Control and Prevention,Nanjing 210009 China
      • 4. Wuhan Center for Disease Control and Prevention,Wuhan 430015,China
      • 5. Hunan Center for Disease Control and Prevention,Changsha 410005,China
      • 6. National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China
      • Received Date: 31 July 2006
      • Accepted Date: 31 August 2006
      Fund Project:  The Key Technologies R & D Programme of the 10th National five-year-plan 2004BA718 b03

      Abstract: A group of 25 rabies viruses (RABVs), recovered from 24 dogs and one human case, were collected from various areas in China between 2004 and 2006. Genetic and phylogenetic analyses of the G-L intergenic region were carried out in 25 street RABV isolates and CTN vaccine strains of 7 generations. The study was based on the comparison of a 519 bp nucleotide sequence, encompassing the G-L intergenic region. The nucleotide sequence homologies of Chinese street strains were from 95.5% to 100%. The phylogenetic analysis showed that all Chinese isolates clearly supported the placement of all Chinese viruses in Lyssavirus genotype 1 and they were distributed according to their geographical origins. All of the Chinese strains were closely related but they could still be divided into two groups: group of street strains and group of CTN strains. This study presents details about the molecular epidemiology of rabies viruses based on the sequences of the G-L Intergenic region.

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