Cloning and Analysis of Partial Genome Sequence of Tobacco vein distorting virus

MO Xiao; QIN Xi; YANG Cheng; DUAN Yu-qi; WU Juan; LI Tian-fei; WU Jian-yu; CHEN Hai-ru

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February 2003

MO Xiao, QIN Xi, YANG Cheng, DUAN Yu-qi, WU Juan, LI Tian-fei, WU Jian-yu and CHEN Hai-ru. Cloning and Analysis of Partial Genome Sequence of Tobacco vein distorting virus[J]. Virologica Sinica, 2003, 18(1): 58-62.

Citation: MO Xiao, QIN Xi, YANG Cheng, DUAN Yu-qi, WU Juan, LI Tian-fei, WU Jian-yu, CHEN Hai-ru. Cloning and Analysis of Partial Genome Sequence of Tobacco vein distorting virus .VIROLOGICA SINICA, 2003, 18(1) : 58-62.

烟草脉扭病毒基因组鄙分序列的克隆和分析

1.
1．云南烟草科学研究院生物技术重点实验室、农业研究所，云南昆明650106
2.
云南农业大学植物病理重点实验室，云南昆明650201
3.
大理烟草科学研究所，云南大理671000

摘要

烟草脉扭病毒(Tobacco vein d~toaing virus， TVDV)是引起烟草丛顶病的两种病毒之一。TVDV被归为黄症病毒科的暂定成员。应用黄症病毒科的通用引物和根据马铃薯卷叶病毒属成员核酸序列设计的简并引物．通过RT-PCR从烟草丛顶病烟株总RNA中扩增到了TVDV基因组的部分序列。序列分析获得了长度为1654 bD的序列，编码推测的TVDV复制酶基因的部分序列、外壳蛋白基因及运动蛋白基因的全部序列。根据这三个基因编码的氨基酸序列构建的分子进化树分析表明，TVDV为黄症病毒科的确定成员。根据其基因间隔区的长度特征和各ORF编码的氨基酸的分子进化分析，我们推测TVDV应当是马铃薯卷叶病毒属的一个新成员。这是TVDV的分子生物学特征的首次报道。
- 烟草丛顶病
- , 烟草脉扭病毒
- , 分子进化树：黄症病毒科：马铃薯卷叶病毒属

Cloning and Analysis of Partial Genome Sequence of Tobacco vein distorting virus

1.
1．Biotechnology Laboratory and Agricultural Institute of Yunnan Academy of Tobacco Science，Kunming 650106， China
2.
Key Laboratory ofPhytopathology，Yunnan Agricultural University,Kunming 650201，China
3.
Dali Tobacco Research Institute，Dali 671000，China

Abstract

Tobacco vein distorting virus(TVDV)is one of the casual agents of tobacco bushy top disease． It was designated a tentative member of the family Luteoviridae． Partial sequence of the genome of rIvDV was amplified by RT-PCR from total RNA of tobacco affected with tobacco bushy top disease，using universal primers of Luteoviruses and degenerate primer of Poleroviruses．The 1 654 bp sequence of rIvDV genome encoded partial putative RNA-dependent RNA polymerase． coat protein and movement protein． Molecular phylogenetic trees were constructed based on the amino acid sequence of these three proteins．indicating that rIvDV should be a definitive memb er of the family Luteoviridae．According to the higher identities of amino acids of ORFs and the similar length of intergenic spacer region of rIvDV with the member of the genus Polerovirus．we proposed that rIvDV was a new member in the genus Polerovirus．Th is is the first report on the molecular properties of TVDV．
- Tobacco bushy top disease
- , Tobacco vein distorting virus
- , Phylogenetic tree

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Analysis of Partial Genome Sequence of Tobacco vein distorting virus

1. 1．Biotechnology Laboratory and Agricultural Institute of Yunnan Academy of Tobacco Science，Kunming 650106， China
2. Key Laboratory ofPhytopathology，Yunnan Agricultural University,Kunming 650201，China
3. Dali Tobacco Research Institute，Dali 671000，China

Abstract: Tobacco vein distorting virus(TVDV)is one of the casual agents of tobacco bushy top disease． It was designated a tentative member of the family Luteoviridae． Partial sequence of the genome of rIvDV was amplified by RT-PCR from total RNA of tobacco affected with tobacco bushy top disease，using universal primers of Luteoviruses and degenerate primer of Poleroviruses．The 1 654 bp sequence of rIvDV genome encoded partial putative RNA-dependent RNA polymerase． coat protein and movement protein． Molecular phylogenetic trees were constructed based on the amino acid sequence of these three proteins．indicating that rIvDV should be a definitive memb er of the family Luteoviridae．According to the higher identities of amino acids of ORFs and the similar length of intergenic spacer region of rIvDV with the member of the genus Polerovirus．we proposed that rIvDV was a new member in the genus Polerovirus．Th is is the first report on the molecular properties of TVDV．