Analysis of Structural Polypeptides and Restriction Patterns of Parocneria orienta Nuclear polyhedrovirus

DIAO Xiao-Dong; ZHOU Jian-Hua; DING Qing-Quan

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June 2000

DIAO Xiao-Dong, ZHOU Jian-Hua and DING Qing-Quan. Analysis of Structural Polypeptides and Restriction Patterns of Parocneria orienta Nuclear polyhedrovirus[J]. Virologica Sinica, 2000, 15(3): 249-254.

Citation: DIAO Xiao-Dong, ZHOU Jian-Hua, DING Qing-Quan. Analysis of Structural Polypeptides and Restriction Patterns of Parocneria orienta Nuclear polyhedrovirus .VIROLOGICA SINICA, 2000, 15(3) : 249-254.

蜀柏毒蛾核型多角体病毒结构多肽及基因组酶切分析

1.
中国科学院武设病毒研究所,武汉430071

摘要

对蜀柏毒蛾核型多角体病毒 (ParocneriaorientaNuclearpolyhedrovirus,简称PaorNPV)形态结构、结构多肽、限制性内切酶图谱等特性进行了研究。采用不连续系统垂直板SDS PAGE分析了PaorNPV的多角体蛋白、病毒粒子结构多肽。应用 5种限制性内切酶对PaorNPV基因组DNA进行了酶切分析。结果表明 :经热处理的多角体蛋白仅有一条带 ,分子量为 31.5kD ,不经热处理的多角体蛋白有三条带 ,分子量分别为 31.5kD、2 9.1kD、2 8.6kD ;病毒粒子包含有 2 5种结构多肽 ,分子量范围在 17.6 114.6kD之间。PaorNPVDNA经BamHI。EcoRI、HindⅢ、PstI和XhoI酶切分别产生 9、12、12、12和 14条片段。基因组大小平均为 12 4 .6kb。
- 蜀柏毒蛾核型多角体病毒
- , 结构多肽
- , 内切酶图谱

Analysis of Structural Polypeptides and Restriction Patterns of Parocneria orienta Nuclear polyhedrovirus

1.
Wuhan Institute of

Abstract

The shape, structure, structural polypeptides and restriction pattern of Parocneria orienta Nuclear polyhedrovirus (PaorNPV)was studied. Assayed by vertical slab SDS PAGE, polyhedrin treated by heat contained a single polypeptide with molecular weight of 31.5 kD, while polyhedrin untreated by heat contained three polypeptides with molecular weight of 31.5 kD, 29.1 kD, 28.6 kD respectively; virion contained 25 structural polypeptides with molecular weight range from 17.6 kD to 114.6 kD. Digestion of PaorNPV DNA with restriction endonucleases, Bam H I, Eco R I, Hin d Ⅲ, Pst I and Xho I, resulted in 9,12,12,12 and 14 fragments, respectively. The PaorNPV genome was about 124.6 kilobasepairs (kb).
- Parocneria orienta Nuclear polyhedrovirus （PaorNPV）
- , Structural polypeptides
- , Restriction endonucleas pattern

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Analysis of Structural Polypeptides and Restriction Patterns of Parocneria orienta Nuclear polyhedrovirus

1. Wuhan Institute of

Abstract: The shape, structure, structural polypeptides and restriction pattern of Parocneria orienta Nuclear polyhedrovirus (PaorNPV)was studied. Assayed by vertical slab SDS PAGE, polyhedrin treated by heat contained a single polypeptide with molecular weight of 31.5 kD, while polyhedrin untreated by heat contained three polypeptides with molecular weight of 31.5 kD, 29.1 kD, 28.6 kD respectively; virion contained 25 structural polypeptides with molecular weight range from 17.6 kD to 114.6 kD. Digestion of PaorNPV DNA with restriction endonucleases, Bam H I, Eco R I, Hin d Ⅲ, Pst I and Xho I, resulted in 9,12,12,12 and 14 fragments, respectively. The PaorNPV genome was about 124.6 kilobasepairs (kb).